Gabrijel Mateja, Bergant Martina, Kreft Marko, Jeras Matjaz, Zorec Robert
Laboratory of Neuroendocrinology-Molecular Cell Physiology, Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia.
J Membr Biol. 2009 May;229(1):11-8. doi: 10.1007/s00232-009-9171-7. Epub 2009 May 6.
Late endocytic compartments, containing MHC class II molecules in antigen presenting cells, fuse to each other in order to deliver antigens to these molecules. We have shown previously that fusion of late endocytic compartments takes place also in hybridomas. Therefore, we investigate here whether the level of fused late endocytic compartments affects the immunostimulatory capacity of hybridomas obtained by the electrofusion of dendritic and tumor cells. The level of fused late endocytic compartments in a single hybridoma cell was assessed and samples of electrofused cells were then cocultured with autologous T cells, resulting in the priming of naïve T cells. To test the immunostimulatory capacity of hybridoma cells, T-cell-induced cytotoxicity of tumor cells was assayed. The results demonstrate that in vitro cytotoxic T cell responses are enhanced if a higher percentage of fused late endocytic compartments is present in the cell population of electrofused hybridoma cells.
在抗原呈递细胞中，含有II类主要组织相容性复合体分子的晚期内吞区室相互融合，以便将抗原递送至这些分子。我们之前已经表明，晚期内吞区室的融合也发生在杂交瘤中。因此，我们在此研究晚期内吞区室的融合水平是否会影响通过树突状细胞与肿瘤细胞电融合获得的杂交瘤的免疫刺激能力。评估单个杂交瘤细胞中晚期内吞区室的融合水平，然后将电融合细胞样本与自体T细胞共培养，从而启动幼稚T细胞。为了测试杂交瘤细胞的免疫刺激能力，检测了T细胞诱导的肿瘤细胞毒性。结果表明，如果电融合杂交瘤细胞群体中存在更高百分比的融合晚期内吞区室，则体外细胞毒性T细胞反应会增强。
