Kato Tomoaki, Tanabe Shigeru, Nishimura Marie, Ohtake Yuko, Nishizawa Yoko, Shimizu Takafumi, Jikumaru Yusuke, Koga Jinichiro, Okada Kazunori, Yamane Hisakazu, Minami Eiichi
Plant-Microbe Interactions Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kan'non dai, Tsukuba, Ibaraki 305-8602, Japan.
Plant Mol Biol. 2009 Aug;70(6):617-25. doi: 10.1007/s11103-009-9495-9. Epub 2009 May 6.
We analyzed the response of rice to Magnaporthe oryzae infection using two mutant strains deficient in Mgb1 and Mst12, which are essential for the development of appresoria and penetration pegs. Both mutants induced the much lower levels of accumulation of phytoalexins than wild-type, suggesting that the massive production of phytoalexins requires the fungal invasion of rice cells. Intense accumulation of H2O2 in a single whole cell also required fungal penetration. Microarray analysis of rice gene expression revealed mutant-specific gene expression, indicating that signal exchange between rice and M. oryzae commence before fungal penetration of the rice cell. In situ detection of mRNAs for peroxidase and beta-1,3-glucanase showed that expression of these genes also occurs after penetration as observed for phytoalexin production.
我们使用两种Mgb1和Mst12缺陷型突变菌株分析了水稻对稻瘟病菌感染的反应，这两种蛋白对于附着胞和侵入栓的形成至关重要。与野生型相比，这两种突变体诱导植保素积累的水平要低得多，这表明植保素的大量产生需要真菌侵入水稻细胞。单个完整细胞中H2O2的强烈积累也需要真菌的穿透。水稻基因表达的微阵列分析揭示了突变体特异性的基因表达，表明水稻和稻瘟病菌之间的信号交换在真菌穿透水稻细胞之前就已开始。过氧化物酶和β-1,3-葡聚糖酶mRNA的原位检测表明，这些基因的表达也在穿透后发生，如同植保素产生时所观察到的那样。
