Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand.
Plant Signal Behav. 2010 Nov;5(11):1353-8. doi: 10.4161/psb.5.11.12950. Epub 2010 Nov 1.
Plant-fungal symbioses are a common feature in nature. They vary from pathogenic interactions, where fungi subvert plant resources for their own use, to mutualistic associations, where both fungus and host benefit from the interaction. Although the ecological importance of plant-fungal symbioses has long been recognized and the biology of several key associations are now well studied, new technologies have the potential to allow fresh insight into the molecular basis of plant-fungal interactions. One such technique - high throughput RNA sequencing - has recently been used to explore the molecular basis of cross-species communications. Here, we give a brief overview of this emerging technology, and present a general guide for employing the methodology to dissect plant-fungal symbiosis.
植物-真菌共生是自然界的普遍特征。它们从病原菌与植物的相互作用，到真菌和宿主都从相互作用中受益的互利共生，形式多样。尽管植物-真菌共生的生态重要性早已得到认可，并且现在对几个关键共生关系的生物学已有了很好的研究，但新技术有可能使人们对植物-真菌相互作用的分子基础有新的认识。其中一种技术 - 高通量 RNA 测序 - 最近已被用于探索跨物种交流的分子基础。在这里，我们简要概述了这项新兴技术，并提供了一个使用该方法剖析植物-真菌共生关系的一般指南。
