Onel Susanne-Filiz, Renkawitz-Pohl Renate
Philipps-Universität Marburg, Fachbereich Biologie, Entwicklungsbiologie, Marburg, Germany.
Dev Dyn. 2009 Jun;238(6):1513-25. doi: 10.1002/dvdy.21961.
In Drosophila, as in mammals, myoblast fusion is fundamental for development. This fusion process has two distinct phases that share common ultrastructural features and at least some molecular players between Drosophila and vertebrates. Here, we integrate the latest data on the key molecular players and ultrastructural features found during myoblast fusion into a new working model to explain this fundamental cellular process. At cell-cell contact sites, a protein complex (FuRMAS) serves as a signalling centre and might restrict the area of membrane fusion. The FuRMAS consists of a ring of cell adhesion molecules, signalling proteins, and F-actin. Regulated F-actin branching plays a pivotal role in myoblast fusion with regard to vesicle transport, fusion pore formation, and expansion as well as the integration of the fusion-competent myoblast into the growing myotube. Interestingly, local F-actin accumulation is a typical feature of other transient adhesive structures such as the immunological synapse, podosomes, and invadopodia. Developmental Dynamics 238:1513-1525, 2009. (c) 2009 Wiley-Liss, Inc.
在果蝇中，与哺乳动物一样，成肌细胞融合对发育至关重要。这种融合过程有两个不同阶段，在果蝇和脊椎动物之间具有共同的超微结构特征以及至少一些分子参与者。在这里，我们将成肌细胞融合过程中发现的关键分子参与者和超微结构特征的最新数据整合到一个新的工作模型中，以解释这一基本的细胞过程。在细胞 - 细胞接触部位，一种蛋白质复合物（FuRMAS）作为信号中心，可能会限制膜融合的区域。FuRMAS由一圈细胞粘附分子、信号蛋白和F - 肌动蛋白组成。在成肌细胞融合过程中，受调控的F - 肌动蛋白分支在囊泡运输、融合孔形成与扩张以及有融合能力的成肌细胞整合到生长中的肌管方面起着关键作用。有趣的是，局部F - 肌动蛋白积累是其他短暂性粘附结构（如免疫突触、足体和侵袭性伪足）的典型特征。《发育动力学》238:1513 - 1525，2009年。（c）2009年威利 - 利斯公司。
