Banerjee Amartya, Saha Achintya, Das Parikshit, Kakati Ajay, Saha Buddhadeb, Goyary Danswrang, Bhutia Yangchen D, Karmakar Sanjeev, Kishor Sumit, Rahaman Saidur, Chattopadhyay Pronobesh
Division of Pharmaceutical Technology, Defence Research Laboratory (DRL), DRDO, Tezpur, Assam, 784001, India.
Department of Chemical Technology, University of Calcutta, Kolkata, India.
Sci Rep. 2024 Apr 22;14(1):9163. doi: 10.1038/s41598-024-59728-7.
Emerging infestations of bed bugs are affecting normal human lifestyle globally. This study has been designed to optimize the rearing conditions for Cimex lectularius L. (Hemiptera), to support the scientific research on them. Bed bugs have been projected onto three different temperature (20 °C, 25 °C, and 30 °C) and relative humidity (50%, 70%, and 90%) conditions to check their overall growth and survival rate. Adult mortality, weight loss, egg laying, percentage hatching, hatching initiation and completion, nymph mortality, and molting have been evaluated to optimize the best conditions. The temperature at 25 °C with 90% RH showed minimum mortality for adults (female 13.33 ± 3.33% and male 6.67 ± 3.33%) and nymphs (13.33 ± 3.33%), while maximum egg laying (40.33 ± 1.86), with highest percentage hatching (98.23 ± 0.58%). At 30 °C with 90% RH, hatching initiation and completion (5.19 ± 0.12 days and 7.23 ± 0.16 days) as well as molting initiation and completion (3.73 ± 0.12 days and 7.00 ± 0.24 days) were found to be fastest. Thus, it can be concluded that 25 °C with 90% RH is ideal for rearing of adults and 30 °C with 90% RH is appropriate for rapid growth of nymphs.
臭虫新出现的侵扰正在影响全球正常的人类生活方式。本研究旨在优化温带臭虫（半翅目）的饲养条件，以支持对其的科学研究。将臭虫置于三种不同的温度（20°C、25°C和30°C）和相对湿度（50%、70%和90%）条件下，以检查它们的整体生长和存活率。对成虫死亡率、体重减轻、产卵、孵化率、孵化起始和完成情况、若虫死亡率以及蜕皮进行了评估，以优化最佳条件。25°C和90%相对湿度的条件下，成虫（雌性13.33±3.33%，雄性6.67±3.33%）和若虫（13.33±3.33%）的死亡率最低，而产卵量最高（40.33±1.86），孵化率也最高（98.23±0.58%）。在30°C和90%相对湿度的条件下，孵化起始和完成时间（5.19±0.12天和7.23±0.16天）以及蜕皮起始和完成时间（3.73±0.12天和7.00±0.24天）最快。因此，可以得出结论，25°C和90%相对湿度是成虫饲养的理想条件，30°C和90%相对湿度适合若虫的快速生长。
