Van den Berg J S
Department of Medicine, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Republic of South Africa.
J S Afr Vet Assoc. 1991 Jun;62(2):48-50.
Clinically normal horses (n = 8) with ages ranging from 5 to 8 years, were starved for 12 h and their plasma ammonia concentrations were measured. The mean fasting plasma ammonia concentration was 17.8 +/- 3.8 mumol l-1. After dosing ammonium chloride at a dose rate of 0.02 g kg-1, there was a significant increase in plasma ammonia concentration, with a maximum rise after 20 min (P less than 0.05). To investigate the influence of temperature on plasma ammonia concentrations of stored samples, 8 plasma samples were stored at -20 degrees C and 4 degrees C respectively. The plasma ammonia concentrations were measured after 6, 12 and 24 h in each of the stored samples. Plasma ammonia concentrations increased significantly after 12 and 24 h when stored at 4 degrees C (P less than 0.05). When plasma was stored at -20 degrees C there was no significant increase from baseline concentrations during 24h (P greater than 0.05).
选取8匹年龄在5至8岁之间临床健康的马匹，使其饥饿12小时后测量其血浆氨浓度。空腹血浆氨浓度的平均值为17.8±3.8微摩尔/升。以0.02克/千克的剂量率给予氯化铵后，血浆氨浓度显著升高，在20分钟后达到最大增幅（P<0.05）。为研究温度对储存样本血浆氨浓度的影响，分别将8份血浆样本储存在-20℃和4℃。在每个储存样本的6小时、12小时和24小时后测量血浆氨浓度。当储存在4℃时，血浆氨浓度在12小时和24小时后显著升高（P<0.05）。当血浆储存在-20℃时，24小时内与基线浓度相比无显著升高（P>0.05）。
