Hitt M E, Jones B D
Am J Vet Res. 1986 Feb;47(2):363-4.
Stability of ammonia in canine plasma was determined, with regard to temperature and time of storage. Heparinized venous blood samples were collected from 8 healthy dogs, immediately placed in an ice water bath, and centrifuged at 5 C. Plasma was harvested from the blood samples, and the initial analysis of each sample for plasma ammonia was performed within 30 minutes after collection. Separate aliquots of the plasma from each dog were stored at 21 C, 4 C, -15 C, or -40 C. Ammonia concentrations of the aliquots stored at the various temperatures were determined at 24, 48, and 96 hours after collection. Statistical analysis of the data from each dog did not indicate a significant relationship between the initial concentration of plasma ammonia and subsequent determinations. Correlation or significance was not found among samples stored at similar temperatures and evaluated at similar times.
针对温度和储存时间，测定了犬血浆中氨的稳定性。从8只健康犬采集肝素化静脉血样本，立即置于冰水浴中，并在5℃下离心。从血样中获取血浆，在采集后30分钟内对每个样本进行血浆氨的初始分析。将每只犬的血浆分别分成若干份，分别储存在21℃、4℃、-15℃或-40℃。在采集后24、48和96小时测定储存在不同温度下的各份血浆的氨浓度。对每只犬的数据进行统计分析，结果表明血浆氨的初始浓度与后续测定之间无显著关系。在相似温度下储存并在相似时间评估的样本之间未发现相关性或显著性。
