Mohanty Swomitra K, Warrick Jay, Gorski Jack, Beebe David J
Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
Electrophoresis. 2009 May;30(9):1470-81. doi: 10.1002/elps.200800595.
We present a rapidly fabricated micro-capillary electrophoresis chip that utilizes surface-tension-driven flow for sample injection and extraction of DNA. Surface-tension-driven flow (i.e. passive pumping) [G. M. Walker et al., Lab. Chip. 2002, 2, 131-134] injects a fixed volume of sample that can be predicted mathematically. Passive pumping eliminates the need for tubing, valves, syringe pumps, and other equipment typically needed for interfacing with microelectrophoresis chips. This method requires a standard micropipette to load samples before separation, and remove the resulting bands after analysis. The device was made using liquid phase photopolymerization to rapidly fabricate the chip without the need of special equipment typically associated with the construction of microelectrophoresis chips (e.g. cleanroom) [A. K. Agarwal et al., J. Micromech. Microeng. 2006, 16, 332-340; S. K. Mohanty et al., Electrophoresis 2006, 27, 3772-3778]. Batch fabrication time for the device presented here was 1.5 h including channel coating time to suppress electroosmotic flow. Devices were constructed out of poly-isobornyl acrylate and glass. A standard microscope with a UV source was used for sample detection. Separations were demonstrated using Promega BenchTop 100 bp ladder in hydroxyl ethyl cellulose (HEC) and oligonucleotides of 91 and 118 bp were used to characterize sample injection and extraction of DNA bands. The end result was an inexpensive micro-capillary electrophoresis device that uses tools (e.g. micropipette, electrophoretic power supplies, and microscopes) already present in most labs for sample manipulation and detection, making it more accessible for potential end users.
我们展示了一种快速制造的微毛细管电泳芯片，该芯片利用表面张力驱动的流动进行样品注入和DNA提取。表面张力驱动的流动（即被动泵送）[G.M.沃克等人，《实验室芯片》，2002年，第2卷，第131 - 134页]注入固定体积的样品，该体积可以通过数学方法预测。被动泵送无需使用通常与微电泳芯片连接所需的 tubing、阀门、注射泵和其他设备。此方法在分离前需要一个标准微量移液器来加载样品，并在分析后去除所得条带。该设备采用液相光聚合快速制造芯片，无需通常与微电泳芯片制造相关的特殊设备（如洁净室）[A.K.阿加瓦尔等人，《微机械与微工程杂志》，2006年，第16卷，第332 - 340页；S.K.莫汉蒂等人，《电泳》，2006年，第27卷，第3772 - 3778页]。此处展示的设备的批量制造时间为1.5小时，包括用于抑制电渗流的通道涂层时间。设备由聚异冰片基丙烯酸酯和玻璃制成。使用带有紫外光源的标准显微镜进行样品检测。使用Promega BenchTop 100 bp梯尺在羟乙基纤维素（HEC）中进行了分离演示，并使用91和118 bp的寡核苷酸来表征DNA条带的样品注入和提取。最终结果是一种廉价的微毛细管电泳设备，它使用大多数实验室中已有的工具（如微量移液器、电泳电源和显微镜）进行样品操作和检测，使其对潜在终端用户更易获取。
