口腔细菌提取物促进人牙髓来源细胞的早期成骨/成牙本质分化。
Oral bacterial extracts facilitate early osteogenic/dentinogenic differentiation in human dental pulp-derived cells.
作者信息
Abe Shu, Imaizumi Mari, Mikami Yoshikazu, Wada Yoshiyuki, Tsuchiya Shuhei, Irie Seiko, Suzuki Shinnosuke, Satomura Kazuhito, Ishihara Kazuyuki, Honda Masaki J
机构信息
Division of Stem Sell Engineering, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Jan;109(1):149-54. doi: 10.1016/j.tripleo.2009.08.028.
OBJECTIVES
Bacterial metabolites demineralize dental hard tissues, and soluble factors lead to tertiary dentinogenesis in the area of the dentin-pulp complex. However, it is unclear whether the oral bacteria are directly involved in the differentiation of dental pulp cells. In this study, we evaluated the effect of oral bacterial extracts on cellular differentiation in human dental pulp-derived cells (hDPC).
STUDY DESIGN
The hDPC were obtained from third molar teeth, and the cells were subcultured. The sonicated extracts were obtained from Porphyromonas gingivalis (gram-negative) and Streptococcus mutans (gram-positive). The effect of bacterial extracts on cellular growth and differentiation in hDPC were tested.
RESULTS
Alkaline phosphatase activity and bone sialoprotein (BSP) gene expression were increased in hDPC exposed to low concentrations of both sonicated extracts, whereas the activity was decreased upon exposure to high concentrations of sonicated extracts from P. gingivalis.
CONCLUSION
This is the first evidence that oral bacteria have a positive effect on cellular differentiation in hPDC.
目的
细菌代谢产物可使牙齿硬组织脱矿，可溶性因子可导致牙本质 - 牙髓复合体区域发生第三期牙本质形成。然而，尚不清楚口腔细菌是否直接参与牙髓细胞的分化。在本研究中，我们评估了口腔细菌提取物对人牙髓来源细胞（hDPC）细胞分化的影响。
研究设计
hDPC取自第三磨牙，细胞进行传代培养。超声提取物取自牙龈卟啉单胞菌（革兰氏阴性菌）和变形链球菌（革兰氏阳性菌）。测试了细菌提取物对hDPC细胞生长和分化的影响。
结果
暴露于低浓度两种超声提取物的hDPC中，碱性磷酸酶活性和骨涎蛋白（BSP）基因表达增加，而暴露于高浓度牙龈卟啉单胞菌超声提取物时，活性降低。
结论
这是口腔细菌对hPDC细胞分化具有积极作用的首个证据。
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