Zhang Xianrong, Liu Huilang, Wu Junfang, Zhang Xu, Liu Maili, Wang Yong
Dept. of Physiology, School of Basic Medical Science, Wuhan University, 185 Donghu Road, Wuhan, 430071, Hubei Province, China.
Neurochem Int. 2009 Jul;54(8):481-7. doi: 10.1016/j.neuint.2009.02.004. Epub 2009 Feb 24.
The metabolic changes in hippocampus, temporal cortex and prefrontal cortex in SD rats along with aging were explored using a metabonomic approach, which based on high resolution "magic angle spinning"(1)H NMR spectroscopy. The metabolite profiles were analyzed by partial least squares-discriminant analysis, and the results showed that the metabolites of the above three brain regions in old rats were dramatically different from that in the adult and young rats. The old rats showed increased myo-inositol and lactate in all of the three brain regions, and decreased N-acetylaspartate in temporal and frontal cortex, Glutamate-GABA level became imbalance in temporal cortex of old rats. In addition, compared with the adult female rats, male rats had higher levels of N-acetylaspartate, taurine, and creatine in temporal or frontal cortex. The age-related metabolic changes may indicate the early functional alterations of neural cells in these brain regions, especially the temporal cortex. The gender-related metabolic changes suggest the significance of the hormonal regulation in brain metabolism. Our work highlights the potential of metabolic profiling to enhance our understanding of biological mechanisms of brain aging.
采用基于高分辨率魔角旋转（1）H NMR光谱的代谢组学方法，探讨了SD大鼠海马、颞叶皮质和前额叶皮质随衰老的代谢变化。通过偏最小二乘判别分析对代谢物谱进行分析，结果表明老年大鼠上述三个脑区的代谢物与成年和幼年大鼠有显著差异。老年大鼠在所有三个脑区的肌醇和乳酸均增加，颞叶和额叶皮质的N-乙酰天门冬氨酸减少，老年大鼠颞叶皮质的谷氨酸-γ-氨基丁酸水平失衡。此外，与成年雌性大鼠相比，雄性大鼠颞叶或额叶皮质的N-乙酰天门冬氨酸、牛磺酸和肌酸水平更高。与年龄相关的代谢变化可能表明这些脑区神经细胞的早期功能改变，尤其是颞叶皮质。与性别相关的代谢变化表明激素调节在脑代谢中的重要性。我们的工作突出了代谢谱分析在增强我们对脑衰老生物学机制理解方面的潜力。
