Wu Hao-Shu, Zhu Di-Feng, Zhou Chang-Xin, Feng Chu-Rui, Lou Yi-Jia, Yang Bo, He Qiao-Jun
Institute of Pharmacology & Toxicology and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 388 Yu-hang-tang Road, Hangzhou, China.
J Ethnopharmacol. 2009 Jun 22;123(2):288-92. doi: 10.1016/j.jep.2009.03.004. Epub 2009 Mar 20.
Acorus calamus L. (AC), family Araceae, have been used in the Indian and Chinese systems of medicine for hundreds of years. The radix of AC is widely used in the therapy of diabetes in traditional folk medicine of America and Indonesia.
To investigate the insulin sensitizing activity and antidiabetic effects of the ethyl acetate fraction of AC (ACE).
Glucose consumption mediated by insulin was detected in L6 rat skeletal muscle cells. Diabetes and its complications related indexes were monitored after orally administrating to genetically obese diabetic C57BL/Ks db/db mice daily for 3 weeks.
ACE (12.5 and 25 microg/ml) increased glucose consumption mediated by insulin in L6 cells (p<0.05 and p<0.01). In db/db mice, ACE (100 mg/kg) significantly reduced serum glucose, triglyceride, reinforce the decrease of total cholesterol caused by rosiglitazone (at least p<0.05), and markedly reduced free fatty acid (FFA) levels and increased adiponectin levels (p<0.01 and p<0.05) as rosiglitazone did (p<0.05 and p<0.001). Serum insulin was decreased but not significantly. In addition, ACE decreased the intake of food and water, and did not increase body weight gain whereas rosiglitazone did.
Owing to the ability of insulin sensitizing, ACE has the potential to be useful for the treatment of diabetes and cardiovascular complications without body weight gain.
菖蒲（Acorus calamus L.，AC），天南星科，在印度和中国医学体系中已被使用数百年。AC的根在美国和印度尼西亚的传统民间医学中广泛用于糖尿病治疗。
研究菖蒲乙酸乙酯部位（ACE）的胰岛素增敏活性和抗糖尿病作用。
在L6大鼠骨骼肌细胞中检测胰岛素介导的葡萄糖消耗。对遗传性肥胖糖尿病C57BL/Ks db/db小鼠每日口服给药3周后，监测糖尿病及其并发症相关指标。
ACE（12.5和25微克/毫升）增加L6细胞中胰岛素介导的葡萄糖消耗（p<0.05和p<0.01）。在db/db小鼠中，ACE（100毫克/千克）显著降低血糖、甘油三酯，增强罗格列酮引起的总胆固醇降低（至少p<0.05），并显著降低游离脂肪酸（FFA）水平，增加脂联素水平（p<0.01和p<0.05），与罗格列酮的作用相同（p<0.05和p<0.001）。血清胰岛素降低但不显著。此外，ACE减少食物和水的摄入量，且不增加体重，而罗格列酮会增加体重。
由于具有胰岛素增敏能力，ACE有潜力用于治疗糖尿病和心血管并发症且不增加体重。
