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来自共生光杆菌的PirAB毒素作为登革热媒介的杀幼虫剂

PirAB toxin from Photorhabdus asymbiotica as a larvicide against dengue vectors.

作者信息

Ahantarig Arunee, Chantawat Nantarat, Waterfield Nicholas R, ffrench-Constant Richard, Kittayapong Pattamaporn

机构信息

Center of Excellence for Vectors and Vector-Borne Diseases, Mahidol University at Salaya, Nakhon Pathom, Thailand.

出版信息

Appl Environ Microbiol. 2009 Jul;75(13):4627-9. doi: 10.1128/AEM.00221-09. Epub 2009 May 8.

DOI:10.1128/AEM.00221-09
PMID:19429564
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2704829/
Abstract

We have evaluated Photorhabdus insect-related protein (Pir) from Photorhabdus asymbiotica against dengue vectors. PirAB shows larvicidal activity against both Aedes aegypti and Aedes albopictus larvae but did not affect the Mesocyclops thermocyclopoides predator. PirAB expressed the strongest toxicity compared to PirA, PirB, or the mixture of PirA plus PirB. Whether the presence of an enterobacterial repetitive intergenic consensus sequence in PirAB, but not in PirA, PirB, or the mixture of PirA plus PirB, has any impact on biological control efficacy needs further investigation.

摘要

我们评估了来自共生光杆状菌的光杆状菌昆虫相关蛋白(Pir)对登革热媒介的作用。PirAB对埃及伊蚊和白纹伊蚊幼虫均表现出杀幼虫活性,但对嗜热中剑水蚤捕食者没有影响。与PirA、PirB或PirA加PirB的混合物相比,PirAB表现出最强的毒性。PirAB中存在肠杆菌重复基因间共有序列,而PirA、PirB或PirA加PirB的混合物中不存在该序列,其是否对生物防治效果有任何影响尚需进一步研究。

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A single Photorhabdus gene, makes caterpillars floppy (mcf), allows Escherichia coli to persist within and kill insects.一个单一的发光杆菌基因,即使毛虫变软(mcf)基因,能让大肠杆菌在昆虫体内存活并杀死昆虫。
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Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.由含有阿拉伯糖PBAD启动子的载体进行严格调控、调节和高水平表达。
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