Serikov Vladimir, Hounshell Catherine, Larkin Sandra, Green William, Ikeda Hirokazu, Walters Mark C, Kuypers Frans A
Children's Hospital Oakland Research Institute, Oakland, CA 94609, USA.
Exp Biol Med (Maywood). 2009 Jul;234(7):813-23. doi: 10.3181/0809-BC-262. Epub 2009 May 8.
The main barrier to a broader clinical application of umbilical cord blood (UCB) transplantation is its limiting cellular content. Thus, the discovery of hematopoietic progenitor cells in murine placental tissue led us investigate whether the human placenta contains hematopoietic cells, sites of hematopoiesis, and to develop a procedure of processing and storing placental hematopoietic cells for transplantation. Here we show that the human placenta contains large numbers of CD34-expressing hematopoietic cells, with the potential to provide a cellular yield several-fold greater than that of a typical UCB harvest. Cells from fresh or cryopreserved placental tissue generated erythroid and myeloid colonies in culture, and also produced lymphoid cells after transplantation in immunodeficient mice. These results suggest that human placenta could become an important new source of hematopoietic cells for allogeneic transplantation.
脐带血（UCB）移植更广泛临床应用的主要障碍是其有限的细胞含量。因此，在小鼠胎盘组织中发现造血祖细胞促使我们研究人类胎盘是否含有造血细胞、造血位点，并开发一种处理和储存胎盘造血细胞用于移植的程序。在这里，我们表明人类胎盘含有大量表达CD34的造血细胞，其细胞产量有可能比典型的脐带血采集量高出几倍。来自新鲜或冷冻保存的胎盘组织的细胞在培养中产生红系和髓系集落，并且在免疫缺陷小鼠中移植后还产生淋巴细胞。这些结果表明，人类胎盘可能成为同种异体移植造血细胞的重要新来源。
