Children's Hospital Oakland Research Institute, Oakland, California 94609, USA.
Stem Cells Transl Med. 2012 May;1(5):359-72. doi: 10.5966/sctm.2011-0021. Epub 2012 May 8.
We describe human chorionic mesenchymal stem cell (hCMSC) lines obtained from the chorion of human term placenta with high therapeutic potential in human organ pathology. hCMSCs propagated for more than 100 doublings without a decrease in telomere length and with no telomerase activity. Cells were highly positive for the embryonic stem cell markers OCT-4, NANOG, SSEA-3, and TRA-1-60. In vitro, cells could be differentiated into neuron-like cells (ectoderm), adipocytes, osteoblasts, endothelial-like cells (mesoderm), and hepatocytes (endoderm)-derivatives of all three germ layers. hCMSCs effectively facilitated repair of injured epithelium as demonstrated in an ex vivo-perfused human lung preparation injured by Escherichia coli endotoxin and in in vitro human lung epithelial cultures. We conclude that the chorion of human term placenta is an abundant source of multipotent stem cells that are promising candidates for cell-based therapies.
我们描述了从人足月胎盘中获得的人绒毛膜间充质干细胞（hCMSC）系，它们在人类器官病理学中具有很高的治疗潜力。hCMSC 经过 100 多次倍增而没有端粒长度的减少，并且没有端粒酶活性。细胞对胚胎干细胞标志物 OCT-4、NANOG、SSEA-3 和 TRA-1-60 呈高度阳性。在体外，细胞可分化为类神经元细胞（外胚层）、脂肪细胞、成骨细胞、内皮样细胞（中胚层）和肝细胞（内胚层）-所有三个胚层的衍生物。hCMSC 有效地促进了受损上皮的修复，这在由大肠杆菌内毒素损伤的体外灌注人肺制备物和体外人肺上皮培养物中得到了证明。我们得出结论，人足月胎盘的绒毛膜是多能干细胞的丰富来源，这些干细胞是细胞治疗的有前途的候选物。
