人胎盘间充质干细胞具有高治疗潜能。
Multipotent stromal stem cells from human placenta demonstrate high therapeutic potential.
机构信息
Children's Hospital Oakland Research Institute, Oakland, California 94609, USA.
出版信息
Stem Cells Transl Med. 2012 May;1(5):359-72. doi: 10.5966/sctm.2011-0021. Epub 2012 May 8.
Abstract
We describe human chorionic mesenchymal stem cell (hCMSC) lines obtained from the chorion of human term placenta with high therapeutic potential in human organ pathology. hCMSCs propagated for more than 100 doublings without a decrease in telomere length and with no telomerase activity. Cells were highly positive for the embryonic stem cell markers OCT-4, NANOG, SSEA-3, and TRA-1-60. In vitro, cells could be differentiated into neuron-like cells (ectoderm), adipocytes, osteoblasts, endothelial-like cells (mesoderm), and hepatocytes (endoderm)-derivatives of all three germ layers. hCMSCs effectively facilitated repair of injured epithelium as demonstrated in an ex vivo-perfused human lung preparation injured by Escherichia coli endotoxin and in in vitro human lung epithelial cultures. We conclude that the chorion of human term placenta is an abundant source of multipotent stem cells that are promising candidates for cell-based therapies.
摘要
我们描述了从人足月胎盘中获得的人绒毛膜间充质干细胞（hCMSC）系，它们在人类器官病理学中具有很高的治疗潜力。hCMSC 经过 100 多次倍增而没有端粒长度的减少，并且没有端粒酶活性。细胞对胚胎干细胞标志物 OCT-4、NANOG、SSEA-3 和 TRA-1-60 呈高度阳性。在体外，细胞可分化为类神经元细胞（外胚层）、脂肪细胞、成骨细胞、内皮样细胞（中胚层）和肝细胞（内胚层）-所有三个胚层的衍生物。hCMSC 有效地促进了受损上皮的修复，这在由大肠杆菌内毒素损伤的体外灌注人肺制备物和体外人肺上皮培养物中得到了证明。我们得出结论，人足月胎盘的绒毛膜是多能干细胞的丰富来源，这些干细胞是细胞治疗的有前途的候选物。
相似文献
1
Multipotent stromal stem cells from human placenta demonstrate high therapeutic potential.人胎盘间充质干细胞具有高治疗潜能。
Stem Cells Transl Med. 2012 May;1(5):359-72. doi: 10.5966/sctm.2011-0021. Epub 2012 May 8.
2
Human multipotent mesenchymal stromal cells from fetal lung expressing pluripotent markers and differentiating into cell types of three germ layers.人胎肺多能间充质基质细胞表达多能标志物，并分化为三个胚层的细胞类型。
Cell Transplant. 2009;18(10):1093-109. doi: 10.3727/096368909X12483162197042. Epub 2009 Jun 22.
3
Ontological differences in first compared to third trimester human fetal placental chorionic stem cells.与第三孕期相比，第一孕期人类胎儿胎盘绒毛膜干细胞的本体论差异。
PLoS One. 2012;7(9):e43395. doi: 10.1371/journal.pone.0043395. Epub 2012 Sep 4.
4
Human chorionic-plate-derived mesenchymal stem cells and Wharton's jelly-derived mesenchymal stem cells: a comparative analysis of their potential as placenta-derived stem cells.人绒毛膜板来源的间充质干细胞和华通氏胶来源的间充质干细胞：作为胎盘来源干细胞的潜能比较分析。
Cell Tissue Res. 2011 Oct;346(1):53-64. doi: 10.1007/s00441-011-1249-8. Epub 2011 Oct 11.
5
Differential mesengenic potential and expression of stem cell-fate modulators in mesenchymal stromal cells from human-term placenta and bone marrow.人足月胎盘和骨髓间充质基质细胞的差异间充质发生潜能和干细胞命运调节因子的表达。
J Cell Physiol. 2012 Sep;227(9):3234-42. doi: 10.1002/jcp.24014.
6
Human chorion-derived stem cells: changes in stem cell properties during serial passage.人绒毛膜干细胞：连续传代过程中干细胞特性的变化。
Cytotherapy. 2011 May;13(5):582-93. doi: 10.3109/14653249.2010.549121. Epub 2011 Jan 13.
7
In vitro differentiation of human placenta-derived multipotent cells into hepatocyte-like cells.人胎盘来源的多能细胞体外分化为肝细胞样细胞。
Stem Cells. 2006 Jul;24(7):1759-68. doi: 10.1634/stemcells.2005-0521.
8
Comparison of Human Amniotic, Chorionic, and Umbilical Cord Multipotent Mesenchymal Stem Cells Regarding Their Capacity for Differentiation Toward Female Germ Cells.人羊膜、绒毛膜和脐带多能间充质干细胞向雌性生殖细胞分化能力的比较
Cell Reprogram. 2017 Feb;19(1):44-53. doi: 10.1089/cell.2016.0035.
9
Isolation and characterization of chorionic mesenchymal stromal cells from human full term placenta.从足月胎盘分离和鉴定绒毛膜间质基质细胞。
J Korean Med Sci. 2012 Aug;27(8):857-63. doi: 10.3346/jkms.2012.27.8.857. Epub 2012 Jul 25.
10
Isolation of multipotent cells from human term placenta.从人足月胎盘中分离多能细胞。
Stem Cells. 2005;23(1):3-9. doi: 10.1634/stemcells.2004-0098.
引用本文的文献
1
Evaluating the therapeutic potential of different sources of mesenchymal stem cells in acute respiratory distress syndrome.评估不同来源的间充质干细胞在急性呼吸窘迫综合征中的治疗潜力。
Stem Cell Res Ther. 2024 Oct 29;15(1):385. doi: 10.1186/s13287-024-03977-w.
2
Human post-implantation blastocyst-like characteristics of Muse cells isolated from human umbilical cord.从人脐带中分离的 Muse 细胞具有人类着床后胚泡样特征。
Cell Mol Life Sci. 2024 Jul 11;81(1):297. doi: 10.1007/s00018-024-05339-4.
3
Characterization of human placenta-derived exosome (pExo) as a potential osteoarthritis disease modifying therapeutic.鉴定人胎盘来源外泌体（pExo）作为潜在的骨关节炎疾病修饰治疗药物。
Arthritis Res Ther. 2023 Nov 28;25(1):229. doi: 10.1186/s13075-023-03219-z.
4
Regulation of Immune Checkpoint Antigen CD276 (B7-H3) on Human Placenta-Derived Mesenchymal Stromal Cells in GMP-Compliant Cell Culture Media.调控免疫检查点抗原 CD276（B7-H3）在符合 GMP 标准的细胞培养介质中人胎盘间充质基质细胞中的表达。
Int J Mol Sci. 2023 Nov 16;24(22):16422. doi: 10.3390/ijms242216422.
5
Small Extracellular Vesicles Derived from Human Chorionic MSCs as Modern Perspective towards Cell-Free Therapy.人绒毛膜间充质干细胞来源的小细胞外囊泡：细胞游离治疗的现代观点。
Int J Mol Sci. 2021 Dec 18;22(24):13581. doi: 10.3390/ijms222413581.
6
Current Status of Cell-Based Therapies for COVID-19: Evidence From Mesenchymal Stromal Cells in Sepsis and ARDS.基于细胞的 COVID-19 疗法的现状：脓毒症和 ARDS 中间充质基质细胞的证据。
Front Immunol. 2021 Oct 1;12:738697. doi: 10.3389/fimmu.2021.738697. eCollection 2021.
7
Differentiation of Cells Isolated from Afterbirth Tissues into Hepatocyte-Like Cells and Their Potential Clinical Application in Liver Regeneration.从胎盘组织中分离的细胞分化为肝细胞样细胞及其在肝脏再生中的潜在临床应用。
Stem Cell Rev Rep. 2021 Apr;17(2):581-603. doi: 10.1007/s12015-020-10045-2. Epub 2020 Sep 25.
8
An human placental vessel perfusion method to study mesenchymal stem/stromal cell migration.一种用于研究间充质干/基质细胞迁移的人胎盘血管灌注方法。
Stem Cell Investig. 2019 Jan 2;6:2. doi: 10.21037/sci.2018.12.03. eCollection 2019.
9
Therapeutic Implications of Human Umbilical Cord Mesenchymal Stromal Cells in Attenuating Influenza A(H5N1) Virus-Associated Acute Lung Injury.人脐带间充质干细胞减轻甲型 H5N1 流感病毒相关急性肺损伤的治疗意义。
J Infect Dis. 2019 Jan 7;219(2):186-196. doi: 10.1093/infdis/jiy478.
10
Placenta-derived multipotent cells have no effect on the size and number of DMH-induced colon tumors in rats.胎盘来源的多能细胞对二甲基肼诱导的大鼠结肠肿瘤的大小和数量没有影响。
Exp Ther Med. 2017 Sep;14(3):2135-2147. doi: 10.3892/etm.2017.4792. Epub 2017 Jul 12.
本文引用的文献
1
Risk factors in the development of stem cell therapy.干细胞治疗发展中的风险因素。
J Transl Med. 2011 Mar 22;9:29. doi: 10.1186/1479-5876-9-29.
2
Fibroblast growth factors: from molecular evolution to roles in development, metabolism and disease.成纤维细胞生长因子：从分子进化到在发育、代谢和疾病中的作用。
J Biochem. 2011 Feb;149(2):121-30. doi: 10.1093/jb/mvq121. Epub 2010 Oct 12.
3
Neurogenic characteristics of placental stem cells in preeclampsia.子痫前期胎盘干细胞的神经发生特性。
Am J Obstet Gynecol. 2010 Oct;203(4):399.e1-7. doi: 10.1016/j.ajog.2010.06.054. Epub 2010 Aug 17.
4
Epithelial repair mechanisms in the lung.肺部上皮细胞修复机制。
Am J Physiol Lung Cell Mol Physiol. 2010 Jun;298(6):L715-31. doi: 10.1152/ajplung.00361.2009. Epub 2010 Apr 2.
5
Mesenchymal stem cells in human placental chorionic villi reside in a vascular Niche.人胎盘绒毛膜绒毛中的间充质干细胞位于血管壁龛中。
Placenta. 2010 Mar;31(3):203-12. doi: 10.1016/j.placenta.2009.12.006. Epub 2010 Jan 13.
6
Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.人胎盘间充质干细胞：分离后干性维持的关键参数
Am J Obstet Gynecol. 2010 Feb;202(2):193.e1-193.e13. doi: 10.1016/j.ajog.2009.10.869. Epub 2009 Dec 24.
7
Human placenta is a potent hematopoietic niche containing hematopoietic stem and progenitor cells throughout development.人胎盘是一个强大的造血微环境，在整个发育过程中都含有造血干细胞和祖细胞。
Cell Stem Cell. 2009 Oct 2;5(4):385-95. doi: 10.1016/j.stem.2009.08.020.
8
Allogeneic human mesenchymal stem cells for treatment of E. coli endotoxin-induced acute lung injury in the ex vivo perfused human lung.异体人骨髓间充质干细胞用于治疗体外灌注人肺中大肠杆菌内毒素诱导的急性肺损伤。
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16357-62. doi: 10.1073/pnas.0907996106. Epub 2009 Aug 31.
9
Transplantation of allogeneic and xenogeneic placenta-derived cells reduces bleomycin-induced lung fibrosis.同种异体和异种胎盘来源细胞的移植可减轻博来霉素诱导的肺纤维化。
Cell Transplant. 2009;18(4):405-22. doi: 10.3727/096368909788809857.
10
Human term placenta as a source of hematopoietic cells.足月人类胎盘作为造血细胞的来源。
Exp Biol Med (Maywood). 2009 Jul;234(7):813-23. doi: 10.3181/0809-BC-262. Epub 2009 May 8.
Zotero 插件