Animal Parasitic Diseases Laboratory, ANRI, ARS, USDA, Beltsville, Maryland 20705-2350, USA.
Mem Inst Oswaldo Cruz. 2009 Mar;104(2):190-5. doi: 10.1590/s0074-02762009000200011.
Until recently, Toxoplasma gondii was considered to be clonal with little genetic variability. In this paper we summarize recent genotyping data from chickens in Brazil, and pigs, lambs and white-tailed deer in the USA, to demonstrate the high genetic diversity and geographical distribution of T. gondii. A total of 149 T. gondii isolates from 13 geographical areas of Brazil and 182 T. gondii isolates from pigs, 53 isolates from sheep and 15 isolates from fetal white-tailed deer from USA were genotyped using the 10 RCFP-PCR genetic markers SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico. Genotyping of 149 T. gondii isolates from free range chickens in Brazil identified 58 genotype groups. No clonal type II lineage was found. Of the 253 isolates from animals from USA, 18 genotypes were identified, predominantly type II. These studies indicate a higher genetic diversity than previously recognized.
直到最近，刚地弓形虫被认为是无性繁殖的，遗传变异性很小。本文总结了来自巴西鸡、美国猪、绵羊和白尾鹿的最近基因分型数据，以证明刚地弓形虫具有高度的遗传多样性和地理分布。从巴西的 13 个地理区域共分离到 149 株刚地弓形虫，从美国的猪中分离到 182 株，从绵羊中分离到 53 株，从胎儿白尾鹿中分离到 15 株，用 10 个 RCFP-PCR 遗传标记 SAG1、SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1 和 Apico 进行基因分型。对巴西自由放养鸡的 149 株刚地弓形虫进行基因分型，鉴定出 58 个基因型组。未发现无性繁殖型 II 谱系。从美国的动物中分离出的 253 株分离物中，鉴定出 18 种基因型，主要为 II 型。这些研究表明遗传多样性高于以前的认识。
