巴西巴伊亚州散养鸡中弓形虫分离株的遗传多样性
Genetic Diversity of Toxoplasma gondii Isolates From Free-Range Chickens In Bahia, Brazil.
作者信息
Rocha Daniele S, Nilsson Mariana G, Maciel Bianca M, Pena Hilda F J, Alves Bruna F, Silva Aristeu V, Gondim Luís F P, Albuquerque George R
机构信息
1 Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
2 Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
出版信息
J Parasitol. 2018 Aug;104(4):377-382. doi: 10.1645/18-9. Epub 2018 May 15.
The genotyping of 25 isolates of Toxoplasma gondii from free-range chickens in the state of Bahia, Brazil, was performed by PCR-restriction fragment length polymorphism using 11 genetic markers: SAG1, 5'+3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3. The analysis revealed 8 genotypes, 3 of which had not been previously reported. Four genotypes were represented by single isolates, whereas the other genotypes were represented by 2 or more isolates. Five isolates showed mixed infections, and 2 of them were identical. None of the clonal types I, II, or III were found, but 2 isolates corresponded to the Brazilian clonal lineage BrIII. There was a single allele for the c22-8 marker. The CS3 marker demonstrated efficiency in the evaluation of virulence in mice. This study reaffirms the diverse genetic variability of T. gondii in Brazil.
采用聚合酶链反应-限制性片段长度多态性方法，利用11个遗传标记：SAG1、5'+3'SAG2、alt.SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1、Apico和CS3，对巴西巴伊亚州散养家禽中分离出的25株刚地弓形虫进行基因分型。分析发现了8种基因型，其中3种此前未见报道。4种基因型由单个分离株代表，而其他基因型由2个或更多分离株代表。5个分离株显示混合感染，其中2个相同。未发现克隆型I、II或III，但有2个分离株对应巴西克隆谱系BrIII。c22-8标记存在单一等位基因。CS3标记在评估小鼠毒力方面显示出有效性。本研究再次证实了巴西刚地弓形虫具有多样的遗传变异性。
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