雀麦花叶病毒的分子量及其他生物物理特性

The Molecular Weight and Other Biophysical Properties of Bromegrass Mosaic Virus.

作者信息

Bockstahler L E, Kaesberg P

出版信息

Biophys J. 1962 Jan;2(1):1-9. doi: 10.1016/s0006-3495(62)86836-2.

DOI:10.1016/s0006-3495(62)86836-2

PMID:19431313

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1366384/

Abstract

Data are presented which show that bromegrass mosaic virus has a particularly low molecular weight and nucleic acid content. A molecular weight of 4.6 x 10(6) was calculated from the sedimentation coefficient, S degrees (20,w) = 86.2S, the diffusion coefficient, D(20,w) = 1.55 x 10(-7) cm(2)/sec., and an assumed partial specific volume, [UNK] = 0.708 ml/gm. The virus has a ribonucleic acid content of 1.0 x 10(6) atomic mass units. Electrophoresis experiments showed that the virus is stable in 0.10 ionic strength buffers in the pH range 3-6. Breakdown of the virus was observed outside this pH range. Some characteristics of the breakdown products are described.

摘要

所呈现的数据表明雀麦花叶病毒具有特别低的分子量和核酸含量。根据沉降系数S°(20,w)=86.2S、扩散系数D(20,w)=1.55×10⁻⁷cm²/秒以及假定的偏比容[UNK]=0.708ml/g计算出分子量为4.6×10⁶。该病毒的核糖核酸含量为1.0×10⁶原子质量单位。电泳实验表明，该病毒在pH值为3 - 6的0.10离子强度缓冲液中稳定。在此pH范围之外观察到病毒分解。描述了分解产物的一些特性。