• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用在细菌系统中表达的 Bromus mosaic virus 重组衣壳蛋白生产的病毒样颗粒。

Virus-Like Particles Produced Using the Brome Mosaic Virus Recombinant Capsid Protein Expressed in a Bacterial System.

机构信息

Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704 Poznan, Poland.

NanoBioMedical Centre, Adam Mickiewicz University, 61-614 Poznan, Poland.

出版信息

Int J Mol Sci. 2021 Mar 18;22(6):3098. doi: 10.3390/ijms22063098.

DOI:10.3390/ijms22063098
PMID:33803568
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8003079/
Abstract

Virus-like particles (VLPs), due to their nanoscale dimensions, presence of interior cavities, self-organization abilities and responsiveness to environmental changes, are of interest in the field of nanotechnology. Nevertheless, comprehensive knowledge of VLP self-assembly principles is incomplete. VLP formation is governed by two types of interactions: protein-cargo and protein-protein. These interactions can be modulated by the physicochemical properties of the surroundings. Here, we used brome mosaic virus (BMV) capsid protein produced in an expression system to study the impact of ionic strength, pH and encapsulated cargo on the assembly of VLPs and their features. We showed that empty VLP assembly strongly depends on pH whereas ionic strength of the buffer plays secondary but significant role. Comparison of VLPs containing tRNA and polystyrene sulfonic acid (PSS) revealed that the structured tRNA profoundly increases VLPs stability. We also designed and produced mutated BMV capsid proteins that formed VLPs showing altered diameters and stability compared to VLPs composed of unmodified proteins. We also observed that VLPs containing unstructured polyelectrolyte (PSS) adopt compact but not necessarily more stable structures. Thus, our methodology of VLP production allows for obtaining different VLP variants and their adjustment to the incorporated cargo.

摘要

病毒样颗粒（VLPs）由于其纳米尺寸、内部腔室的存在、自组织能力以及对环境变化的响应能力，在纳米技术领域引起了人们的兴趣。然而，对 VLP 自组装原理的全面了解并不完整。VLP 的形成受两种类型的相互作用控制：蛋白-货物和蛋白-蛋白。这些相互作用可以通过周围环境的物理化学性质来调节。在这里，我们使用在表达系统中产生的大麦黄花叶病毒（BMV）衣壳蛋白来研究离子强度、pH 值和包裹货物对 VLPs 组装及其特性的影响。我们表明，空 VLP 组装强烈依赖于 pH 值，而缓冲液的离子强度则起着次要但重要的作用。含有 tRNA 和聚苯乙烯磺酸（PSS）的 VLPs 的比较表明，结构上的 tRNA 极大地增加了 VLPs 的稳定性。我们还设计并生产了突变的 BMV 衣壳蛋白，这些蛋白形成的 VLPs 与由未修饰蛋白组成的 VLPs 相比，其直径和稳定性发生了改变。我们还观察到，含有无规聚合物电解质（PSS）的 VLPs 采用紧凑但不一定更稳定的结构。因此，我们的 VLP 生产方法允许获得不同的 VLP 变体，并对其进行整合货物的调整。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/d7f42b88ece6/ijms-22-03098-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/0346229dc08b/ijms-22-03098-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/a7c339d90ddf/ijms-22-03098-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/0a229e84992c/ijms-22-03098-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/d722e342e565/ijms-22-03098-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/ebe2c7e8800a/ijms-22-03098-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/9f6e854051a0/ijms-22-03098-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/6041b86832c6/ijms-22-03098-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/7980386f5723/ijms-22-03098-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/90975e531f84/ijms-22-03098-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/d7f42b88ece6/ijms-22-03098-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/0346229dc08b/ijms-22-03098-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/a7c339d90ddf/ijms-22-03098-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/0a229e84992c/ijms-22-03098-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/d722e342e565/ijms-22-03098-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/ebe2c7e8800a/ijms-22-03098-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/9f6e854051a0/ijms-22-03098-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/6041b86832c6/ijms-22-03098-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/7980386f5723/ijms-22-03098-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/90975e531f84/ijms-22-03098-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de6e/8003079/d7f42b88ece6/ijms-22-03098-g010.jpg

相似文献

1
Virus-Like Particles Produced Using the Brome Mosaic Virus Recombinant Capsid Protein Expressed in a Bacterial System.使用在细菌系统中表达的 Bromus mosaic virus 重组衣壳蛋白生产的病毒样颗粒。
Int J Mol Sci. 2021 Mar 18;22(6):3098. doi: 10.3390/ijms22063098.
2
Cryo-EM reconstructions of BMV-derived virus-like particles reveal assembly defects in the icosahedral lattice structure.冷冻电镜重建的 BMV 衍生病毒样颗粒揭示了二十面体晶格结构中的组装缺陷。
Nanoscale. 2022 Feb 24;14(8):3224-3233. doi: 10.1039/d1nr05650f.
3
Unravelling the Stability and Capsid Dynamics of the Three Virions of Brome Mosaic Virus Assembled Autonomously .解析组装自主形成的三种雀麦花叶病毒粒子的稳定性和衣壳动力学。
J Virol. 2020 Mar 31;94(8). doi: 10.1128/JVI.01794-19.
4
Phosphorylation of the Brome Mosaic Virus Capsid Regulates the Timing of Viral Infection.雀麦花叶病毒衣壳的磷酸化作用调控病毒感染的时机。
J Virol. 2016 Aug 12;90(17):7748-60. doi: 10.1128/JVI.00833-16. Print 2016 Sep 1.
5
Packaging of tobacco mosaic virus subgenomic RNAs by Brome mosaic virus coat protein exhibits RNA controlled polymorphism.烟草花叶病毒亚基因组RNA被雀麦花叶病毒外壳蛋白包装呈现出RNA控制的多态性。
Virology. 2000 Sep 30;275(2):249-57. doi: 10.1006/viro.2000.0532.
6
Plant Virus-Like Particles for RNA Delivery.植物病毒样颗粒用于 RNA 递呈。
Methods Mol Biol. 2024;2822:387-410. doi: 10.1007/978-1-0716-3918-4_24.
7
Production of VP3-only virus-like particles of Adeno-associated virus 2 in E. coli cells.在大肠杆菌细胞中生产腺相关病毒 2 的仅 VP3 病毒样颗粒。
Protein Expr Purif. 2024 Aug;220:106502. doi: 10.1016/j.pep.2024.106502. Epub 2024 May 15.
8
The coat protein leads the way: an update on basic and applied studies with the Brome mosaic virus coat protein.外壳蛋白引领方向：关于雀麦花叶病毒外壳蛋白基础与应用研究的最新进展。
Mol Plant Pathol. 2011 May;12(4):403-12. doi: 10.1111/j.1364-3703.2010.00678.x. Epub 2010 Nov 25.
9
Self-assembly of virus-like particles of canine parvovirus capsid protein expressed from Escherichia coli and application as virus-like particle vaccine.从大肠杆菌表达的犬细小病毒衣壳蛋白的病毒样颗粒的自组装及其作为病毒样颗粒疫苗的应用。
Appl Microbiol Biotechnol. 2014 Apr;98(8):3529-38. doi: 10.1007/s00253-013-5485-6. Epub 2014 Jan 12.
10
Essential elements of the capsid protein for self-assembly into empty virus-like particles of hepatitis E virus.戊型肝炎病毒衣壳蛋白自组装成空病毒样颗粒的必需元件。
J Virol. 2005 Oct;79(20):12999-3006. doi: 10.1128/JVI.79.20.12999-13006.2005.

引用本文的文献

1
Single-particle studies of the effects of RNA-protein interactions on the self-assembly of RNA virus particles.单颗粒研究 RNA-蛋白相互作用对 RNA 病毒颗粒自组装的影响。
Proc Natl Acad Sci U S A. 2022 Sep 27;119(39):e2206292119. doi: 10.1073/pnas.2206292119. Epub 2022 Sep 19.

本文引用的文献

1
Physics of viral dynamics.病毒动力学的物理学
Nat Rev Phys. 2021;3(2):76-91. doi: 10.1038/s42254-020-00267-1. Epub 2021 Jan 12.
2
How a Virus Circumvents Energy Barriers to Form Symmetric Shells.病毒如何规避能量障碍形成对称外壳。
ACS Nano. 2020 Mar 24;14(3):3170-3180. doi: 10.1021/acsnano.9b08354. Epub 2020 Mar 2.
3
Evolving by deleting: patterns of molecular evolution of isolates from Poland.从波兰分离株看分子进化的模式：删除进化。
J Gen Virol. 2019 Oct;100(10):1442-1456. doi: 10.1099/jgv.0.001290. Epub 2019 Aug 19.
4
Nonequilibrium self-assembly dynamics of icosahedral viral capsids packaging genome or polyelectrolyte.无平衡自组装动力学的二十面体病毒衣壳包装基因组或聚电解质。
Nat Commun. 2018 Aug 6;9(1):3071. doi: 10.1038/s41467-018-05426-8.
5
Synthetic approaches to construct viral capsid-like spherical nanomaterials.构建病毒衣壳样球形纳米材料的合成方法。
Chem Commun (Camb). 2018 Aug 9;54(65):8944-8959. doi: 10.1039/c8cc03844a.
6
Oligonucleotide Length-Dependent Formation of Virus-Like Particles.寡核苷酸长度依赖性病毒样颗粒的形成。
Chemistry. 2018 May 23;24(29):7456-7463. doi: 10.1002/chem.201800285. Epub 2018 Apr 27.
7
The effect of RNA stiffness on the self-assembly of virus particles.RNA 刚性对病毒颗粒自组装的影响。
J Phys Condens Matter. 2018 Jan 31;30(4):044002. doi: 10.1088/1361-648X/aaa159.
8
Biophysical analysis of BMV virions purified using a novel method.使用一种新方法纯化的BMV病毒粒子的生物物理分析。
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Nov 15;1068-1069:157-163. doi: 10.1016/j.jchromb.2017.10.022. Epub 2017 Oct 12.
9
Design of virus-based nanomaterials for medicine, biotechnology, and energy.用于医学、生物技术和能源领域的病毒基纳米材料设计。
Chem Soc Rev. 2016 Jul 25;45(15):4074-126. doi: 10.1039/c5cs00287g.
10
Viruses, Artificial Viruses and Virus-Based Structures for Biomedical Applications.病毒、人工病毒和基于病毒的生物医学应用结构。
Adv Healthc Mater. 2016 Jun;5(12):1386-400. doi: 10.1002/adhm.201501000. Epub 2016 Apr 27.