Christensen Kristi M, Rorrer Gregory L
Oregon State University, Corvallis, 97331, USA.
Chemosphere. 2009 Aug;76(8):1135-42. doi: 10.1016/j.chemosphere.2009.04.018. Epub 2009 May 9.
The partitioning behavior of the polycyclic aromatic hydrocarbon (PAH) compounds naphthalene and phenanthrene with the temperate green seaweed Acrosiphonia coalita was characterized. The uptake and partitioning experiments were designed to prevent PAH volatilization, and the PAH concentration was measured in both the seawater liquid medium and in the algal biomass. Axenic microplantlets of A. coalita were used in all experiments to eliminate the possibility of microbial PAH biotransformation. Gas chromatography/mass spectrometry analysis did not reveal any putative metabolites of phenanthrene oxidative biotransformation in either the seawater medium or the algal biomass, but did show that dissolved organic matter from algal biomass constituents were in the liquid medium. The algal biomass grew by 30% over the 114h duration of the partitioning experiments, suggesting PAH compounds did not harm the organism. Both living and heat-killed microplantlets partitioned PAH compounds into the biomass. Naphthalene and phenanthrene reversibly partitioned into the lipid fraction of the algal biomass with equilibrium partitioning constants of 0.130+/-0.007 and 1.58+/-0.03Lg(-1) dry cell mass, respectively, which scaled proportionally to their octanol-water partitioning constants. The PAH material balance for the partitioning process closed between 86% and 100% for naphthalene adsorption and phenanthrene desorption, but closed at 52% for phenanthrene adsorption. To account for the loss, it was proposed that phenanthrene interacted with dissolved organic matter released by the living algal biomass. This study has provided fundamental information needed to assess how seaweeds can play a role in the bioaccumulation and bioremediation of PAH compounds in the marine environment.
对多环芳烃化合物萘和菲在温带绿藻煤生顶管藻中的分配行为进行了表征。摄取和分配实验旨在防止多环芳烃挥发，并测量了海水液体介质和藻类生物量中的多环芳烃浓度。在所有实验中均使用了煤生顶管藻的无菌微幼苗，以消除微生物对多环芳烃进行生物转化的可能性。气相色谱/质谱分析未在海水介质或藻类生物量中发现菲氧化生物转化的任何假定代谢产物，但确实表明藻类生物量成分中的溶解有机物存在于液体介质中。在分配实验的114小时期间，藻类生物量增长了30%，这表明多环芳烃化合物不会对生物体造成伤害。活的和热灭活的微幼苗都将多环芳烃化合物分配到生物量中。萘和菲可逆地分配到藻类生物量的脂质部分，平衡分配常数分别为0.130±0.007和1.58±0.03Lg（-1）干细胞质量，这与它们的正辛醇-水分配常数成比例缩放。萘吸附和菲解吸的分配过程的多环芳烃物质平衡在86%至100%之间闭合，但菲吸附的闭合率为52%。为了解释这种损失，有人提出菲与活藻类生物量释放的溶解有机物相互作用。这项研究提供了评估海藻如何在海洋环境中多环芳烃化合物的生物积累和生物修复中发挥作用所需的基础信息。
