Muñoz R, Guieysse B, Mattiasson B
Department of Biotechnology, Lund University, P.O. Box 124, 22100, Lund, Sweden.
Appl Microbiol Biotechnol. 2003 May;61(3):261-7. doi: 10.1007/s00253-003-1231-9. Epub 2003 Feb 20.
An algal-bacterial consortium formed by Chlorella sorokiniana and a phenanthrene-degrading Pseudomonas migulae strain was able to biodegrade 200-500 mg/l of phenanthrene dissolved in silicone oil or tetradecane under photosynthetic conditions and without any external supply of oxygen. Phenanthrene was only removed when provided in organic solvent, which confirms the potential of two-phase systems for toxicity reduction. Phenanthrene was degraded at highest rates when provided in silicone oil rather than in tetradecane since this solvent probably sequestered the PAH, reducing its mass transfer to the aqueous phase. The influence of phenanthrene concentration, amount of inoculum and light intensity on pollutant removal was also investigated and, under the best conditions, phenanthrene was degraded at 24.2 g m(-3).h(-1). In addition to being cost-effective and mitigating the release of greenhouse gases into the atmosphere, photosynthetic oxygenation was especially beneficial to the use of two-phase partitioning bioreactors since it prevented solvent emulsification and/or volatilization and evidence was found that the microalgae release biosurfactants that could further enhance phenanthrene degradation.
由索氏小球藻和一株降解菲的迁移假单胞菌组成的藻菌联合体，能够在光合条件下且无需任何外部供氧的情况下，对溶解在硅油或十四烷中的200 - 500毫克/升菲进行生物降解。菲只有在以有机溶剂形式提供时才会被去除，这证实了两相系统在降低毒性方面的潜力。当菲以硅油而非十四烷形式提供时，其降解速率最高，因为这种溶剂可能会螯合多环芳烃，减少其向水相的传质。还研究了菲浓度、接种量和光照强度对污染物去除的影响，在最佳条件下，菲的降解速率为24.2克·米⁻³·小时⁻¹。除了具有成本效益和减少温室气体向大气中的排放外，光合充氧对两相分配生物反应器的使用特别有益，因为它可防止溶剂乳化和/或挥发，并且有证据表明微藻会释放生物表面活性剂，这可进一步增强菲的降解。
