Lee Seo Young, Kim Geun Tae, Roh Si Hun, Song Jin-Su, Kim Hie-Joon, Hong Soon-Sun, Kwon Sung Won, Park Jeong Hill
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea.
Pharmazie. 2009 Apr;64(4):242-7.
Ginseng is a representative herbal medicine in Asia and various pharmacological activities of ginsenoside Rd isolated from ginseng have been reported. However, anti-cancer activity and mechanism of ginsenoside Rd in HT29 colon cancer cell lines were not studied yet. We performed proteomic analysis through two-dimensional gel electrophoresis, MALDI-TOF/TOF-MS and database to identify altered protein induced by ginsenoside Rd treatment in HT29. We can identify fourteen proteins contributed to cell growth inhibition induced by Rd. Proteins involved in the inhibition of mitosis (Stathmin1, Microtubule-associated protein RP/EB family and Stratifin) were significantly up- and down-regulated. And proteins associated with apoptosis (Rho GDP dissociation inhibitor, Tropomyosin1 and Annexin5) were significantly changed. Furthermore, ginsenoside Rd in HT29 was involved in cytoprotection, DNA replication and repair, protein synthesis and degradation, metastasis and mutagenesis. It was supposed that ginsenoside Rd contributed to induce anti-cancer activity by complementary functions of these proteins in colon cancer cells.
人参是亚洲一种具有代表性的草药，从人参中分离出的人参皂苷Rd的多种药理活性已有报道。然而，人参皂苷Rd在HT29结肠癌细胞系中的抗癌活性及机制尚未得到研究。我们通过二维凝胶电泳、基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF-MS）和数据库进行蛋白质组学分析，以鉴定人参皂苷Rd处理HT29细胞后诱导变化的蛋白质。我们能够鉴定出14种有助于Rd诱导细胞生长抑制的蛋白质。参与有丝分裂抑制的蛋白质（Stathmin1、微管相关蛋白RP/EB家族和Stratifin）显著上调和下调。与细胞凋亡相关的蛋白质（Rho GDP解离抑制剂、原肌球蛋白1和膜联蛋白5）也有显著变化。此外，HT29细胞中的人参皂苷Rd还参与细胞保护、DNA复制与修复、蛋白质合成与降解、转移和诱变。推测人参皂苷Rd通过这些蛋白质在结肠癌细胞中的互补功能诱导抗癌活性。
