Department of Food Science & Nutrition, Dankook University, Yongin 448-701, Korea.
J Ginseng Res. 2011 Sep;35(3):315-24. doi: 10.5142/jgr.2011.35.3.315.
This study investigated the effect of red ginseng extract on metastasis of colon cancer cells in vitro and in vivo. Wound healing migration, cell motility, invasion, and activity, protein expression, and mRNA expression of matrix metalloproteinases (MMPs) were examined in SW480 human colon cancer cells. SW480 cells were cultured with or without 100 μg/L PMA in the absence or presence of various concentrations (100, 200, or 300 μg/mL) of red ginseng extract. Red ginseng extract treatment caused significant suppression of cell motility and invasion (p<0.05) in SW480 cells. Red ginseng extract inhibited MMP-2 and MMP-9 activity and their protein and mRNA expression in a dose-dependent manner (p<0.05) in SW480 cells. For experimental metastasis, BALB/c mice were injected intravenously with CT-26 mouse colon cancer cells in the tail vein, and were orally administered various concentrations (0, 75, 150, or 300 mg/kg body weight) of red ginseng extract for 3 weeks. Numbers of pulmonary nodules were significantly decreased in mice that were fed red ginseng extract (p<0.05). Plasma MMP-2 and MMP-9 activity significantly decreased in response to treatment with red ginseng extract in mice (p<0.05). These data suggest that red ginseng extract may be useful for prevention of cancer invasion and metastasis through inhibition of MMP-2 and MMP-9 pathways.
本研究旨在探讨红参提取物对结肠癌 SW480 细胞体外和体内转移的影响。通过划痕愈合迁移实验、细胞迁移、侵袭和活性实验，检测了基质金属蛋白酶（MMPs）的蛋白表达和 mRNA 表达。在无或有不同浓度（100、200 或 300μg/ml）红参提取物的情况下，将 PMA（100μg/L）添加到 SW480 人结肠癌细胞中进行培养。红参提取物处理可显著抑制 SW480 细胞的迁移和侵袭（p<0.05）。红参提取物以剂量依赖的方式抑制 MMP-2 和 MMP-9 的活性及其蛋白和 mRNA 表达（p<0.05）。在实验性转移中，通过尾静脉向 BALB/c 小鼠注射 CT-26 结肠癌细胞，并在 3 周内口服给予红参提取物的不同浓度（0、75、150 或 300mg/kg 体重）。红参提取物喂养的小鼠肺部结节数显著减少（p<0.05）。红参提取物处理后，小鼠血浆 MMP-2 和 MMP-9 活性显著降低（p<0.05）。这些数据表明，红参提取物可能通过抑制 MMP-2 和 MMP-9 途径，有助于预防癌症的侵袭和转移。
