Anil Kumar Pinnaka, Naga Radha Srinivas Tanuku, Sasikala Chintalapati, Venkata Ramana Chintalapati, Süling Jorg, Imhoff Johannes
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Hyderabad, India.
J Gen Appl Microbiol. 2009 Apr;55(2):163-9. doi: 10.2323/jgam.55.163.
A green sulfur bacterium, strain JAGS6T was isolated from a marine aquaculture pond located near Kakinada on the east coast of India. Cells of strain JAGS6T were Gram-negative, non-motile, coccoid, 1-1.2 microm in diameter, with prosthecae. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JAGS6T clusters with members of the genus Prosthecochloris and the sequence similarity with the nearest relative, Prosthecochloris vibrioformis, is 96.7%. Cultures of strain JAGS6T are green in color and the cells contain bacteriochlorophyll c and most likely carotenoids of the chlorobactene series as photosynthetic pigments. Strain JAGS6T is mesophilic, halotolerant (up to 7% NaCl) and is obligately phototrophic, utilizing sulfide but not thiosulfate as a photosynthetic electron donor. Sulfur globules are deposited outside the cells during oxidation of sulfide. On the basis of 16S rRNA gene sequence analysis and its morphological and physiological characteristics, strain JAGS6T is distinct from described species of the genus Prosthecochloris and we propose to describe it as a new species, Prosthecochloris indica, sp. nov. The type strain is JAGS6T (=JCM 13299T=ATCC BAA1214T).
从印度东海岸卡基纳达附近的一个海水养殖池塘中分离出一株绿色硫细菌，菌株JAGS6T。菌株JAGS6T的细胞革兰氏阴性，无运动性，呈球状，直径1-1.2微米，有菌柄。基于16S rRNA基因序列的系统发育分析表明，菌株JAGS6T与Prosthecochloris属的成员聚类，与最接近的亲缘种Prosthecochloris vibrioformis的序列相似性为96.7%。菌株JAGS6T的培养物呈绿色，细胞含有细菌叶绿素c，最有可能含有氯菌烯系列的类胡萝卜素作为光合色素。菌株JAGS6T是嗜温的，耐盐（高达7% NaCl），专性光合自养，利用硫化物而非硫代硫酸盐作为光合电子供体。在硫化物氧化过程中，硫球沉积在细胞外。基于16S rRNA基因序列分析及其形态和生理特征，菌株JAGS6T与已描述的Prosthecochloris属物种不同，我们建议将其描述为一个新物种，印度Prosthecochloris，新种。模式菌株为JAGS6T（=JCM 13299T=ATCC BAA1214T）。
