Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 33, bld. 2 Leninsky Ave, Moscow, 119071, Russia.
, Pr. 60‑letiya Oktyabrya 7 k. 2, Moscow, 117312, Russia.
Arch Microbiol. 2019 Dec;201(10):1399-1404. doi: 10.1007/s00203-019-01707-y. Epub 2019 Jul 23.
A Gram-negative, anaerobic photoautotroph, nonmotile, oval bacterium possessing gas vesicles and having no prosthecae, designated as V1, was isolated from the South China Sea coastal zone. It had chlorosomes as photosynthetic structures, and bacteriochlorophyll c as the major photosynthetic pigment. The strain was found to grow at 20-35 °C, pH 6.3-8.0 (optimum, pH 7.1) and with 0.7-5.8% (w/v) NaCl (optimum, 1-1.8%). In the presence of sulfide and bicarbonate, acetate, and fructose promoted growth. The DNA G+C content was 47 mol%. While the new isolate belonged to the Chlorobiaceae genus Prosthecochloris, it exhibited low similarity of the 16S rRNA gene sequences (96.21-96.78%) to other members of this genus. Comparison of the genome nucleotide sequences of strain V1 revealed that the new isolate was remote from the Chlorobiaceae type strains both in dDDH (16.8-18.9%) and in ANI (75.2-77.8%). We propose to assign the isolate to a new species, Prosthecochloris marina sp. nov., with the type strain V1 ( = VKM-3301 = KCTC 15824).
一种革兰氏阴性、厌氧的光自养菌，不运动，呈椭圆形，具有气泡囊但没有菌毛，被命名为 V1，从中国南海沿海地区分离得到。它具有作为光合作用结构的菌绿体和主要的光合色素细菌叶绿素 c。该菌株被发现可以在 20-35°C、pH6.3-8.0（最佳，pH7.1）和 0.7-5.8%（w/v）NaCl（最佳，1-1.8%）的条件下生长。在存在硫化物和碳酸氢盐的情况下，乙酸盐和果糖促进生长。DNA G+C 含量为 47 mol%。虽然新分离株属于 Chlorobiaceae 属 Prosthecochloris，但它的 16S rRNA 基因序列（96.21-96.78%）与该属的其他成员相似度较低。比较菌株 V1 的基因组核苷酸序列表明，新分离株与 Chlorobiaceae 型菌株在 dDDH（16.8-18.9%）和 ANI（75.2-77.8%）方面都存在较远的关系。我们建议将该分离株分配到一个新的物种 Prosthecochloris marina sp. nov. 中，其模式菌株为 V1（ = VKM-3301 = KCTC 15824）。
