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Determination and location of phosphoserine in proteins and peptides by conversion to S-ethylcysteine.

作者信息

Meyer H E, Hoffmann-Posorske E, Heilmeyer L M

出版信息

Methods Enzymol. 1991;201:169-85. doi: 10.1016/0076-6879(91)01016-u.

DOI:10.1016/0076-6879(91)01016-u
PMID:1943763
Abstract
摘要

相似文献

1
Determination and location of phosphoserine in proteins and peptides by conversion to S-ethylcysteine.通过转化为S-乙基半胱氨酸来测定蛋白质和肽中磷酸丝氨酸的含量及位置
Methods Enzymol. 1991;201:169-85. doi: 10.1016/0076-6879(91)01016-u.
2
Analysis of sites of protein phosphorylation.蛋白质磷酸化位点分析
Methods Mol Biol. 1997;64:293-306. doi: 10.1385/0-89603-353-8:293.
3
Sequence analysis of phosphotyrosine-containing peptides.含磷酸酪氨酸肽段的序列分析
Methods Enzymol. 1991;201:206-24. doi: 10.1016/0076-6879(91)01019-x.
4
Quantitative determination of phosphoserine by high-performance liquid chromatography as the phenylthiocarbamyl-S-ethylcysteine. Application to picomolar amounts of peptides and proteins.通过高效液相色谱法将磷酸丝氨酸定量测定为苯硫代甲酰基-S-乙基半胱氨酸。应用于皮摩尔量的肽和蛋白质。
J Chromatogr. 1987 Jun 26;397:113-21. doi: 10.1016/s0021-9673(01)84994-3.
5
Separation of phosphotyrosine, phosphoserine, and phosphothreonine by high-performance liquid chromatography.通过高效液相色谱法分离磷酸酪氨酸、磷酸丝氨酸和磷酸苏氨酸。
Methods Enzymol. 1991;201:3-10. doi: 10.1016/0076-6879(91)01003-k.
6
Cardiac troponin I, isolated from bovine heart, contains two adjacent phosphoserines. A first example of phosphoserine determination by derivatization to S-ethylcysteine.
Eur J Biochem. 1988 Sep 15;176(2):335-42. doi: 10.1111/j.1432-1033.1988.tb14286.x.
7
Synthesis of O-phosphoserine- and O-phosphothreonine-containing peptides.含O-磷酸丝氨酸和O-磷酸苏氨酸的肽的合成。
Methods Enzymol. 1991;201:225-33. doi: 10.1016/0076-6879(91)01020-3.
8
Solid-phase sequencing of 32P-labeled phosphopeptides at picomole and subpicomole levels.
Methods Enzymol. 1991;201:186-99. doi: 10.1016/0076-6879(91)01017-v.
9
Quantitative and selective fluorophore labeling of phosphoserine on peptides and proteins: characterization at the attomole level by capillary electrophoresis and laser-induced fluorescence.肽和蛋白质上磷酸丝氨酸的定量及选择性荧光团标记:通过毛细管电泳和激光诱导荧光在阿托摩尔水平进行表征。
Anal Biochem. 1995 Feb 10;225(1):81-8. doi: 10.1006/abio.1995.1111.
10
Synthesis of phosphopeptides containing O-phosphoserine or O-phosphothreonine.含O-磷酸丝氨酸或O-磷酸苏氨酸的磷酸肽的合成。
Int J Pept Protein Res. 1989 Jun;33(6):468-76. doi: 10.1111/j.1399-3011.1989.tb00225.x.

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Recent development of analytical methods for disease-specific protein -GlcNAcylation.疾病特异性蛋白质O-连接N-乙酰葡糖胺化分析方法的最新进展。
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O-GlcNAc profiling: from proteins to proteomes.O-GlcNAc 谱分析:从蛋白质到蛋白质组。
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Selective detection of phosphopeptides in complex mixtures by electrospray liquid chromatography/mass spectrometry.电喷雾液相色谱/质谱法对复杂混合物中磷酸肽的选择性检测。
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4
Identification of protein phosphorylation sites by a combination of mass spectrometry and solid phase Edman sequencing.通过质谱法和固相埃德曼测序相结合的方法鉴定蛋白质磷酸化位点。
J Biomol Tech. 2002 Sep;13(3):119-30.
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Molecular biologist's guide to proteomics.蛋白质组学分子生物学家指南
Microbiol Mol Biol Rev. 2002 Mar;66(1):39-63; table of contents. doi: 10.1128/MMBR.66.1.39-63.2002.
6
cAMP-dependent protein kinase inhibits mGluR2 coupling to G-proteins by direct receptor phosphorylation.环磷酸腺苷(cAMP)依赖性蛋白激酶通过直接使受体磷酸化来抑制代谢型谷氨酸受体2(mGluR2)与G蛋白的偶联。
J Neurosci. 2000 Aug 1;20(15):5663-70. doi: 10.1523/JNEUROSCI.20-15-05663.2000.
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Proteophosphoglycans of Leishmania mexicana. Identification, purification, structural and ultrastructural characterization of the secreted promastigote proteophosphoglycan pPPG2, a stage-specific glycoisoform of amastigote aPPG.墨西哥利什曼原虫的蛋白磷酸聚糖。分泌型前鞭毛体蛋白磷酸聚糖pPPG2的鉴定、纯化、结构和超微结构表征,pPPG2是无鞭毛体aPPG的阶段特异性糖异构体。
Biochem J. 1999 Dec 15;344 Pt 3(Pt 3):775-86. doi: 10.1042/bj3440775.
8
Insulin-stimulated kinase from rat fat cells that phosphorylates initiation factor 4E-binding protein 1 on the rapamycin-insensitive site (serine-111).来自大鼠脂肪细胞的胰岛素刺激激酶,其可在雷帕霉素不敏感位点(丝氨酸-111)使起始因子4E结合蛋白1磷酸化。
Biochem J. 1998 Nov 15;336 ( Pt 1)(Pt 1):39-48. doi: 10.1042/bj3360039.
9
A model for the function of the bisphosphorylated heart-specific troponin-I N-terminus.双磷酸化心脏特异性肌钙蛋白-I N 端功能的模型。
J Muscle Res Cell Motil. 1998 Aug;19(6):647-59. doi: 10.1023/a:1005381131102.
10
Domains of tau protein, differential phosphorylation, and dynamic instability of microtubules.tau蛋白结构域、磷酸化差异与微管的动态不稳定性
Mol Biol Cell. 1995 Dec;6(12):1887-902. doi: 10.1091/mbc.6.12.1887.