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Cardiac troponin I, isolated from bovine heart, contains two adjacent phosphoserines. A first example of phosphoserine determination by derivatization to S-ethylcysteine.

作者信息

Swiderek K, Jaquet K, Meyer H E, Heilmeyer L M

机构信息

Abteilung für Biochemie Supramolekularer Systeme, Ruhr-Universitt Bochum, Federal Republic of Germany.

出版信息

Eur J Biochem. 1988 Sep 15;176(2):335-42. doi: 10.1111/j.1432-1033.1988.tb14286.x.

DOI:10.1111/j.1432-1033.1988.tb14286.x
PMID:3138117
Abstract

Bovine cardiac troponin containing approximately 3 mol P/mol protein could be separated into its subunits without loss of phosphate. Troponin I and troponin T each contain about 1.5 mol P/mol protein. In troponin I two phosphorylated serine residues could be localized in the N-terminal region by conversion of phosphoserine to S-ethylcysteine. They are located in adjacent positions in the following sequence: -Arg-Arg-Ser(P)-Ser(P)-Ala-Asn-Tyr-Tyr-Arg-Ala-Tyr-Ala-Thr-Glu-Pro- His-Ala-Lys. This sequence shows that the first phosphoserine residue in bovine cardiac troponin I occupies a homologous position to phosphoserine-20 of rabbit cardiac troponin I.

摘要

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