Waguespack A M, Powell S, Bidner T D, Payne R L, Southern L L
School of Animal Sciences, Louisiana State University Agricultural Center, Baton Rouge 70803, USA.
Poult Sci. 2009 Jun;88(6):1216-26. doi: 10.3382/ps.2008-00452.
Research was conducted to determine the level of l-Lys that can be included in corn-soybean meal (C-SBM) diets for broilers before an amino acid (AA) beyond Met, Lys, Thr, or Gly becoming limiting and to determine the order of limiting AA in low CP C-SBM diets. All experiments were conducted with Ross 708 broilers (0 to 18 d of age) in brooder batteries. Treatments contained 7 or 8 replicates with 6 birds per replicate. In all experiments, a control C-SBM diet containing no l-Lys.HCl and a similar diet [positive control (PC) + Gly] with supplemental Gly to provide 2.32% total dietary Gly + Ser were fed. All diets were formulated to contain 1.26% total Lys. All diets with added l-Lys.HCl contained supplemental Gly to provide 2.32% total dietary Gly + Ser. In experiment 1, l-Lys.HCl was added to the diets at 0.02% increments from 0.15 to 0.27%. Compared with the PC + Gly diet, there were no negative effects (P > 0.10) of supplemental Lys on ADG, ADFI, or G:F. In experiment 2, l-Lys.HCl was added to the diets at 0.05% increments from 0.25 to 0.60%. Compared with the PC + Gly diet, ADG and G:F were decreased (P < 0.03) in broilers fed diets containing greater than 0.30% l-Lys.HCl but not (P > 0.10) in the 0.25% l-Lys.HCl diet. In experiment 3, l-Lys.HCl was added to the diets at 0.05% increments from 0.20 to 0.30%. Daily gain was decreased (P < 0.03) in broilers fed 0.30% l-Lys.HCl but not in those fed 0.20 or 0.25% l-Lys.HCl. In experiment 4, the order of limiting AA was determined in a C-SBM diet containing 0.45% L-Lys.HCl. In addition to the PC and PC + Gly diets, diets consisted of a negative control (NC) diet with 0.45% l-Lys.HCl, NC + 0.247% Ile, NC + 0.484% l-Arg.HCl, NC + 0.249% Val, and all possible 2- and l-way combinations of all 3 AA. Compared with the NC diet, addition of Arg and the combination of Arg and the other AA increased ADG and ADFI, indicating that Arg was the limiting AA in this diet. Experiment 5 was conducted in an identical manner to experiment 4 except the diets with the added AA contained the same ratio of corn to soybean meal that is present in a diet with 0.25% l-Lys.HCl. The results of experiment 5 suggest that Arg and Val are equaling limiting in a diet with 0.25% l-Lys.HCl. In summary, 0.25% l-Lys.HCl can be added to C-SBM diets supplemented with Met, Thr, and Gly with no negative effects on growth performance, and Arg and Val are equaling limiting (after Met, Thr, Lys, and Gly) in diets containing 0.25% l-Lys.HCl.
开展本研究旨在确定在蛋氨酸、赖氨酸、苏氨酸或甘氨酸以外的氨基酸成为限制氨基酸之前，可添加到肉仔鸡玉米 - 豆粕（C - SBM）日粮中的L - 赖氨酸水平，并确定低粗蛋白C - SBM日粮中限制氨基酸的顺序。所有试验均使用罗斯708肉仔鸡（0至18日龄），饲养于育雏笼中。处理组包含7或8个重复，每个重复6只鸡。在所有试验中，饲喂不含L - 盐酸赖氨酸的对照C - SBM日粮以及添加甘氨酸以提供2.32%总日粮甘氨酸 + 丝氨酸的类似日粮[阳性对照（PC）+ 甘氨酸]。所有日粮均配制为含1.26%总赖氨酸。所有添加L - 盐酸赖氨酸的日粮均添加甘氨酸以提供2.32%总日粮甘氨酸 + 丝氨酸。在试验1中，以0.02%的增量向日粮中添加L - 盐酸赖氨酸，添加量从0.15%至0.27%。与PC + 甘氨酸日粮相比，添加赖氨酸对平均日增重（ADG）、平均日采食量（ADFI）或料重比（G:F）无负面影响（P > 0.10）。在试验2中，以0.05%的增量向日粮中添加L - 盐酸赖氨酸，添加量从0.25%至0.60%。与PC + 甘氨酸日粮相比，饲喂含大于0.30% L - 盐酸赖氨酸日粮的肉仔鸡ADG和G:F降低（P < 0.03），但在含0.25% L - 盐酸赖氨酸的日粮中未降低（P > 0.10）。在试验3中，以0.05%的增量向日粮中添加L - 盐酸赖氨酸，添加量从0.20%至0.30%。饲喂0.30% L - 盐酸赖氨酸日粮的肉仔鸡日增重降低（P < 0.03），但饲喂0.20%或0.25% L - 盐酸赖氨酸日粮的肉仔鸡未降低。在试验4中，确定了含0.45% L - 盐酸赖氨酸的C - SBM日粮中限制氨基酸的顺序。除了PC和PC + 甘氨酸日粮外，日粮还包括含0.45% L - 盐酸赖氨酸的阴性对照（NC）日粮、NC + 0.247%异亮氨酸、NC + 0.484% L - 盐酸精氨酸、NC + 单249%缬氨酸，以及所有3种氨基酸的所有可能的二元和三元组合。与NC日粮相比，添加精氨酸以及精氨酸与其他氨基酸的组合增加了ADG和ADFI，表明精氨酸是该日粮中的限制氨基酸。试验5的进行方式与试验4相同，只是添加氨基酸的日粮中玉米与豆粕的比例与含0.25% L - 盐酸赖氨酸的日粮相同。试验5的结果表明，在含0.25% L - 盐酸赖氨酸的日粮中，精氨酸和缬氨酸的限制作用相当。总之，可向补充了蛋氨酸、苏氨酸和甘氨酸的C - SBM日粮中添加0.25% L - 盐酸赖氨酸，且对生长性能无负面影响，在含0.25% L - 盐酸赖氨酸的日粮中，精氨酸和缬氨酸的限制作用相当（仅次于蛋氨酸、苏氨酸、赖氨酸和甘氨酸）。
