Department of Animal Sciences, University of Illinois, Urbana, USA.
Evonik Operations GmbH, Nutrition & Care, Hanau-Wolfgang, Germany.
Poult Sci. 2021 Mar;100(3):100971. doi: 10.1016/j.psj.2020.12.068. Epub 2020 Dec 29.
Production of crystalline amino acids (AA) through microbial fermentation concomitantly provides an AA-enriched biomass that may serve as a cost-effective supplement for broiler chickens. We investigated the effects of feeding a fermentation biomass product containing approximately 62% Lys on growth performance, organ growth, and clinical outcomes of broilers. Beginning at 2 d post-hatch, a total of 360 Ross 308 chicks were randomly assigned to 1 of 5 treatments provided to 12 replicate cages of 6 birds. Practical corn-soybean meal-based dietary treatments included: negative control (NC; no supplementation of L-Lys, 1.01 and 0.86% standardized ileal digestible Lys in starter and grower phases, respectively), NC + 0.23% L-Lys HCl (positive control; PC), and NC supplemented with 0.30, 0.90, or 1.50% Lys biomass (LB) in both phases. Feed and water were provided ad libitum throughout the study. Individual bird and feeder weights were recorded on study day 0, 10, 21, and 35. At study conclusion, birds from each treatment were randomly selected to collect blood and tissue samples. The PC and 0.30% LB diets elicited similar overall (day 0-35) body weight gain and birds were heavier (P < 0.001) than the NC and other LB treatments. The PC, 0.30% LB, and 0.90% LB groups had better (P < 0.001) overall feed conversion ratio than NC. Some LB-supplemented treatments elicited increased (P < 0.001) relative spleen and ileum weight compared with NC and PC. Heterophils were increased (P < 0.001) in LB treatments compared with PC and NC. Lymphocytes were decreased (P < 0.001) in LB treatments compared with NC, and 1.50% LB was similar to PC. This resulted in an increased (P < 0.001) heterophil-to-lymphocyte ratio in some LB treatments, which may have resulted from general AA supplementation or the LB product. Collectively, these results suggest that addition of up to 0.30% LB restored growth performance when added to a Lys-deficient practical diet and elicited results identical to the Lys-adequate PC diet with no negative clinical effects.
通过微生物发酵生产结晶氨基酸 (AA) 同时提供了富含 AA 的生物质，可作为肉仔鸡的经济有效的补充饲料。我们研究了在 2 日龄后给肉鸡饲喂含有约 62%Lys 的发酵生物质产品对其生长性能、器官生长和临床结果的影响。共有 360 只罗斯 308 雏鸡随机分配到 5 种处理中的 1 种，12 个重复笼，每个笼 6 只鸡。实用的玉米-豆粕基础日粮处理包括：负对照（NC；不补充 L-Lys，在起始和生长阶段分别为 1.01%和 0.86%标准回肠可消化 Lys）、NC+0.23%L-Lys HCl（正对照；PC）和 NC 分别在两个阶段补充 0.30%、0.90%或 1.50%Lys 生物质（LB）。在整个研究期间，鸡可以自由采食饲料和饮水。在研究的第 0、10、21 和 35 天记录鸡的个体体重和料位体重。研究结束时，从每个处理中随机选择鸡采集血液和组织样本。PC 和 0.30%LB 日粮引起的总体（0-35 天）体重增加相似，鸡的体重比 NC 和其他 LB 处理组重（P<0.001）。PC、0.30%LB 和 0.90%LB 组的总体饲料转化率优于 NC（P<0.001）。与 NC 和 PC 相比，一些 LB 处理组的脾脏和回肠相对重量增加（P<0.001）。与 PC 和 NC 相比，LB 处理组的异嗜白细胞增加（P<0.001）。与 NC 相比，LB 处理组的淋巴细胞减少（P<0.001），而 1.50%LB 与 PC 相似。这导致一些 LB 处理组的异嗜白细胞与淋巴细胞的比值增加（P<0.001），这可能是由于一般 AA 补充或 LB 产品所致。总的来说，这些结果表明，在添加到缺乏 Lys 的实用日粮中时，添加高达 0.30%的 LB 可以恢复生长性能，并产生与 Lys 充足的 PC 日粮相同的结果，没有任何临床负面影响。
