Hamaguchi-Hamada Kayoko, Fujisawa Yuko, Koizumi Osamu, Muneoka Youjiro, Okado Nobuo, Hamada Shun
Department of Nutrition and Health Science, Faculty of Human Environmental Science, Fukuoka Women's University, Higashi-Ku, Fukuoka, 813-8529, Japan.
Cell Tissue Res. 2009 Jul;337(1):15-25. doi: 10.1007/s00441-009-0808-8. Epub 2009 May 14.
The GLW-amide family is a neuropeptide family found in cnidarian species and is characterized by the C-terminal amino acid sequence -Gly-Leu-Trp-NH(2). To detect mammalian peptides structurally related to the GLW-amide family, we examined rat brain by immunohistochemistry with an anti-GLW-amide antibody. GLW-amide-like immunoreactivity (GLW-amide-LI) was observed in thin varicose fibers in some regions of the brain. Most neurons showing GLW-amide-LI were observed in the laterodorsal tegmental nucleus, pedunculopontine tegmental nucleus, and trigeminal/spinal ganglia. These results strongly suggest that the rat nervous system contains as yet unidentified GLW-amide-like peptides, and that GLW-amide-LI in the brain is a good marker for ascending projections from mesopontine cholinergic neurons.
GLW-酰胺家族是在刺胞动物物种中发现的一种神经肽家族，其特征在于C端氨基酸序列-Gly-Leu-Trp-NH₂。为了检测与GLW-酰胺家族在结构上相关的哺乳动物肽，我们用抗GLW-酰胺抗体通过免疫组织化学方法检测了大鼠大脑。在大脑某些区域的细曲张纤维中观察到了GLW-酰胺样免疫反应性（GLW-酰胺-LI）。在外侧背侧被盖核、脚桥被盖核以及三叉神经/脊髓神经节中观察到了大多数显示GLW-酰胺-LI的神经元。这些结果有力地表明，大鼠神经系统中含有尚未鉴定的GLW-酰胺样肽，并且大脑中的GLW-酰胺-LI是中脑桥胆碱能神经元上行投射的良好标志物。
