Yum S, Takahashi T, Koizumi O, Ariura Y, Kobayakawa Y, Mohri S, Fujisawa T
Department of Developmental Genetics, National Institute of Genetics, Shizuoka, Japan.
Biochem Biophys Res Commun. 1998 Jul 30;248(3):584-90. doi: 10.1006/bbrc.1998.8831.
During the course of a systematic non-targeting screening of peptide signal molecules in Hydra, we identified a novel myoactive neuropeptide called Hym-176. The primary structure of Hym-176 was determined to be APFIFPGPKVamide. It specifically and reversibly induced contraction of the ectodermal muscle of the hydra body column in vivo. However, it had no effect on the ectodermal muscle of the tentacles. The structure-activity relationship analysis showed that the sequence of FIFPGPKVamide is a minimal requirement for the myoactivity. Removal of an amide group from the C-terminus completely abolished the activity. By using the antibody specific to Hym-176, the tissue localization of the peptide in hydra was determined immunohistochemically. The intense immunoreactivity was found in the peduncle nerve cells, indicating that Hym-176 is a neuropeptide.
在对水螅中的肽信号分子进行系统性非靶向筛选的过程中，我们鉴定出一种名为Hym-176的新型肌肉活性神经肽。Hym-176的一级结构被确定为APFIFPGPKVamide。它在体内特异性且可逆地诱导水螅体柱外胚层肌肉收缩。然而，它对触手的外胚层肌肉没有影响。结构-活性关系分析表明，FIFPGPKVamide序列是肌肉活性的最低要求。从C末端去除酰胺基团会完全消除活性。通过使用针对Hym-176的特异性抗体，免疫组织化学确定了该肽在水螅中的组织定位。在柄部神经细胞中发现了强烈的免疫反应性，表明Hym-176是一种神经肽。
