Gonçalves L S A, Rodrigues R, do Amaral Júnior A T, Karasawa M, Sudré C P
Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil.
Genet Mol Res. 2009 Mar 31;8(1):364-74. doi: 10.4238/vol8-1gmr549.
Accessions in gene banks need to be characterized and evaluated to determine their genetic diversity. We made a joint diversity analysis of the tomato gene bank of the Universidade Estadual do Norte Fluminense Darcy Ribeiro in Rio de Janeiro state, using the Ward-modified location model. Forty Solanum lycopersicum accessions were characterized and evaluated for 22 morphoagronomic descriptors and 131 random amplified polymorphic DNA markers. Based on the pseudo-F and pseudo-t(2) criteria, the optimal number of groups was established as five. Variability within groups was high for both continuous and discrete nominal data. The first two canonical variables explained about 90% of the inter-group variability. Care should be taken in using the Ward-modified location model technique to avoid incorporating excessive and unnecessary markers, which could favor molecular markers when compared with morphoagronomic variables. However, the minimum number of markers is germplasm- dependent and must be recalculated for each new divergence analysis.
基因库中的种质需要进行特征鉴定和评估，以确定其遗传多样性。我们使用沃德修正位置模型，对里约热内卢州北弗卢米嫩塞州立大学达西·里贝罗分校的番茄基因库进行了联合多样性分析。对40份番茄种质进行了特征鉴定，并针对22个形态农艺性状描述符和131个随机扩增多态性DNA标记进行了评估。基于伪F和伪t(2)标准，确定最佳组数为5组。连续和离散名义数据在组内的变异性都很高。前两个典型变量解释了约90%的组间变异性。在使用沃德修正位置模型技术时应谨慎，以避免纳入过多和不必要的标记，与形态农艺变量相比，这可能有利于分子标记。然而，标记的最小数量取决于种质，并且每次新的差异分析都必须重新计算。
