一种基于新型随机扩增多态性DNA的番茄遗传多样性分析与鉴定策略。

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes.

作者信息

Cao X, Wu Z, Zhou R, Jiang F L, Yang Z E

机构信息

Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China, Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, China.

Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China, Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, China

出版信息

Genet Mol Res. 2015 Mar 6;14(1):1650-61. doi: 10.4238/2015.March.6.11.

DOI:10.4238/2015.March.6.11

PMID:25867307

Abstract

Cultivar identification diagrams (CIDs) provide a rapid and efficient approach for identifying cultivars based on random amplified polymorphic DNA (RAPD) markers. In this paper, 64 tomato cultivars were identified using a CID. Using RAPD profiles, clustering analysis was performed to analyze genetic diversity. The results showed that 8 RAPD primers could completely separate the 64 cultivars according to the obtained polymorphic bands; a CID of the 64 tomato cultivars was then constructed. As verification of the CID validity, 8 randomly selected cultivars were investigated and proven to be well distinguished. In addition, 33 DNA bands were obtained, 20 (60.6%) of which were polymorphic. Genetic distances were calculated with a range of 0.032 to 1.402. Clustering analysis showed that the 64 tomato cultivars were divided into 4 groups with a similarity coefficient of 0.40. Using this novel strategy, with the same RAPD data, both CID and clustering analysis can simultaneously determine tomato cultivars and their genetic diversity.

摘要

品种鉴定图（CID）为基于随机扩增多态性DNA（RAPD）标记鉴定品种提供了一种快速有效的方法。本文利用CID对64个番茄品种进行了鉴定。利用RAPD图谱进行聚类分析以分析遗传多样性。结果表明，8条RAPD引物可根据获得的多态性条带将64个品种完全区分开；随后构建了64个番茄品种的CID。作为对CID有效性的验证，对随机选择的8个品种进行了研究，结果证明它们能够被很好地区分。此外，获得了33条DNA条带，其中20条（60.6%）具有多态性。计算得到的遗传距离范围为0.032至1.402。聚类分析表明，64个番茄品种被分为4组，相似系数为0.40。使用这种新策略，利用相同的RAPD数据，CID和聚类分析都可以同时确定番茄品种及其遗传多样性。