Yilmaz Zeycan, Celik Ismail
Yüzüncü Yil University, Faculty of Science & Letters, Department of Biology, 65080 Van, Turkey.
Neurotoxicology. 2009 May;30(3):382-5. doi: 10.1016/j.neuro.2009.03.002. Epub 2009 Mar 20.
This study was carried out to investigate the neurotoxic and immunotoxic effects of Indole-3-butyric acid (IBA), a plant growth regulator (PGR), on rats at subacute and subchronic exposure. The neurotoxic effects of IBA were evaluated by measuring the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Biomarkers selected for immunotoxic monitoring were the activities of adenosine deaminase (ADA) and myeloperoxidase (MPO) in various tissues of rats exposed to 25 and 50 ppm dosages of IBA for 20 and 45 days. Results showed that the administrations of IBA decreased AChE and BChE activities in some tissues of the rats treated with both dosages and periods of IBA. With regard to the immunotoxic effects, ADA activity significantly decreased whereas MPO activity increased after subacute and subchronic exposure with both dosages in most of the tissues of rats compared with controls. The observations presented led us to conclude that the administrations of IBA at subacute and subchronic exposure decreased AChE, BChE and ADA activities whereas increased MPO activity in various tissues of rats. This may reflect the potential role of these parameters as useful biomarkers for toxicity of IBA.
本研究旨在调查植物生长调节剂吲哚 - 3 - 丁酸（IBA）在亚急性和亚慢性暴露条件下对大鼠的神经毒性和免疫毒性作用。通过测量乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的活性来评估IBA的神经毒性作用。选择腺苷脱氨酶（ADA）和髓过氧化物酶（MPO）的活性作为免疫毒性监测的生物标志物，对暴露于25 ppm和50 ppm剂量IBA 20天和45天的大鼠各组织进行检测。结果表明，在两种剂量和不同暴露时长的IBA处理组大鼠的某些组织中，IBA的施用降低了AChE和BChE的活性。关于免疫毒性作用，与对照组相比，在亚急性和亚慢性暴露于两种剂量IBA的大鼠的大多数组织中，ADA活性显著降低，而MPO活性增加。上述观察结果使我们得出结论，亚急性和亚慢性暴露条件下施用IBA会降低大鼠各组织中AChE、BChE和ADA的活性，而增加MPO的活性。这可能反映了这些参数作为IBA毒性有用生物标志物的潜在作用。
