Isik Ismail, Celik Ismail
Department of Biology, Faculty of Science, Yuzuncu Yil University, Van, Turkey
Department of Biology, Faculty of Science, Yuzuncu Yil University, Van, Turkey.
Toxicol Ind Health. 2015 Dec;31(12):1095-105. doi: 10.1177/0748233713487247. Epub 2013 May 1.
The present study was aimed to investigate the effects of subacute and subchronic treatment of some plant growth regulators (PGRs), such as abscisic acid (ABA) and gibberellic acid (GA3), on neurological and immunological biomarkers in various tissues of rats. The activities of acetylcholinesterase (AChE) and butrylcholinesterase (BChE) were selected as biomarkers for neurotoxic biomarkers. Adenosine deaminase (ADA) and myeloperoxidase (MPO) were measured as indicators for immunotoxic investigation purpose. Wistar albino rats were orally administered with 25 and 50 ppm of PGRs ad libitum for 25-50 days continuously with drinking water. The treatment of PGRs caused different effects on the activities of enzymes. Results showed that the administrations of ABA and GA3 increased AChE and BChE activities in some tissues of rats treated with both the dosages and periods of ABA and GA3. With regard to the immunotoxic effects, ADA activity fluctuated, while MPO activity increased after subacute and subchronic exposure of treated rat tissues to both dosages when compared with the controls. The observations presented led us to conclude that the administrations of PGRs at subacute and subchronic exposure increased AChE, BChE, and MPO activities, while fluctuating the ADA activity in various tissues of rats. This may reflect the potential role of these parameters as useful biomarkers for toxicity of PGRs.
本研究旨在探讨某些植物生长调节剂（PGR），如脱落酸（ABA）和赤霉素（GA3）的亚急性和亚慢性处理对大鼠各组织神经和免疫生物标志物的影响。选择乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的活性作为神经毒性生物标志物。测定腺苷脱氨酶（ADA）和髓过氧化物酶（MPO）作为免疫毒性研究指标。将Wistar白化大鼠自由饮用含25 ppm和50 ppm PGR的饮用水，连续25 - 50天。PGR处理对酶活性产生了不同影响。结果表明，ABA和GA3的给药在一定剂量和处理时间下均增加了大鼠某些组织中AChE和BChE的活性。关于免疫毒性作用，与对照组相比，经亚急性和亚慢性暴露处理的大鼠组织在两种剂量下，ADA活性波动，而MPO活性增加。上述观察结果使我们得出结论，亚急性和亚慢性暴露下PGR的给药增加了大鼠各组织中AChE、BChE和MPO的活性，同时使ADA活性波动。这可能反映了这些参数作为PGR毒性有用生物标志物的潜在作用。
