Uhnáková B, Petrícková A, Biedermann D, Homolka L, Vejvoda V, Bednár P, Papousková B, Sulc M, Martínková L
Institute of Microbiology, Academy of Sciences, 142 20 Prague, Czech Republic.
Chemosphere. 2009 Aug;76(6):826-32. doi: 10.1016/j.chemosphere.2009.04.016. Epub 2009 May 13.
2-Bromophenol (1), 4-bromophenol (2), 2,4-dibromophenol (3), 2,6-dibromophenol (4), 2,4,6-tribromophenol (5) and tetrabromobisphenol A (6) (1 mM each) added to growing submerged cultures of Trametes versicolor CCBAS 612 were eliminated by 65-85% from the culture medium within 4d. Extracellular laccase activity in the culture medium was influenced by the type of brominated compound added. Maximum level of laccase (63 U L(-1)) was found in the culture with 2-bromophenol. Tetrabromobisphenol A was degraded by a commercial laccase from Trametes versicolor in absence of any oxidation mediator, hydroxylated dibrominated compounds being detected as soluble reaction products by LC/MS. A significant degradation of brominated phenols by laccase was achieved only in the presence of ABTS structural characterization of major products suggesting reaction between bromophenol and ABTS radicals.
将2-溴苯酚（1）、4-溴苯酚（2）、2,4-二溴苯酚（3）、2,6-二溴苯酚（4）、2,4,6-三溴苯酚（5）和四溴双酚A（6）（各1 mM）添加到云芝Trametes versicolor CCBAS 612正在生长的深层培养物中，4天内培养基中65%-85%的这些物质被去除。培养基中的胞外漆酶活性受所添加溴化化合物类型的影响。在添加2-溴苯酚的培养物中发现漆酶的最高水平（63 U L(-1)）。在没有任何氧化介质的情况下，商业云芝漆酶可降解四溴双酚A，通过液相色谱/质谱检测到羟基化二溴化化合物作为可溶性反应产物。仅在存在ABTS的情况下，漆酶才能显著降解溴化酚，主要产物的结构表征表明溴苯酚与ABTS自由基之间发生了反应。
