Iakorski P, Kuntsova M M, Loseva E F, Khasanov F K
Mol Gen Mikrobiol Virusol. 1991 Jun(6):19-21.
The gene coding for alpha-amylase from the ruminant bacterium Streptococcus bovis was cloned on the plasmid pMX39 in Bacillus subtilis cells. An alpha-amylase positive colony was isolated in the initial screening of 3900 colonies on the medium containing insoluble starch. The size of the insert was approximately 2.8 kb. The recombinant plasmid was stably maintained in Bacillus subtilis cells under the nonselective conditions.
将反刍动物细菌牛链球菌编码α-淀粉酶的基因克隆到枯草芽孢杆菌细胞中的质粒pMX39上。在含有不溶性淀粉的培养基上对3900个菌落进行初步筛选时，分离出一个α-淀粉酶阳性菌落。插入片段的大小约为2.8 kb。重组质粒在非选择性条件下能在枯草芽孢杆菌细胞中稳定维持。
