通过不稳定淀粉酶活性区分的牛瘤胃链球菌菌株中α-淀粉酶基因的定位

Location of the alpha-amylase gene in rumen Streptococcus bovis strains distinguished by unstable amylase activity.

作者信息

Mareková M, Jonecová Z, Kmeĭ V

机构信息

Institute of Animal Physiology, Slovak Academy of Sciences, Kosice.

出版信息

Folia Microbiol (Praha). 1995;40(2):181-4. doi: 10.1007/BF02815419.

DOI:10.1007/BF02815419

PMID:8851562

Abstract

Genetic stability of amylase activity after serial subcultivation experiments with amylolytic ruminal Streptococcus bovis strains was investigated. Two strains Amy+ and Amy- were obtained. Loss of amylase activity connected with the loss of plasmid DNA was not found in these strains. The presence of the gene responsible for the amylase activity in the chromosome of these strains was revealed by hybridization of the alpha-amylase gene on pJK108 against chromosomal DNA of S. bovis and Bacillus subtilis after a complete restriction with EcoRI.

摘要

通过对具有淀粉分解能力的牛瘤胃链球菌菌株进行连续传代培养实验，研究了淀粉酶活性的遗传稳定性。获得了两个菌株，即Amy+和Amy-。在这些菌株中未发现淀粉酶活性的丧失与质粒DNA的丧失有关。在用EcoRI完全酶切后，通过将pJK108上的α-淀粉酶基因与牛链球菌和枯草芽孢杆菌的染色体DNA进行杂交，揭示了这些菌株染色体中负责淀粉酶活性的基因的存在。