Chen Q, Wu L, Jiang R, Zhang Y, Geng Y
Laboratory of Biotechnology, Nankai University, Tianjin.
Yi Chuan Xue Bao. 1993;20(3):272-8.
The plasmid pE194 as a vector is used to subclone the thermostable alpha-amylase gene of B. licheniformis and construct recombinant plasmid pNW102. The plasmid pNW102 was transduced into B. subilis BF 7658 by bacteriophage PBS1. The B. subtilis BF7658 (pNW102) strain was treated at non-permissive temperature for a long time, and obtained many recombinant strains. This is the homologous recombination results between alpha-amylase genes of pNW102 and BF7658. The homologous recombination occurs at any site in alpha-amylase gene but there are some heat-spots. We have already screened 2 strains from the recombination strains which can stably produce the thermostable alpha-amylase in B. subtilis BF7658. The analysis of enzymology shows that the characters of the thermpstable alpha-amylase produced from the recombinants strains are the same with that of B. licheniformis.
以质粒pE194作为载体，亚克隆地衣芽孢杆菌的耐热α-淀粉酶基因，并构建重组质粒pNW102。通过噬菌体PBS1将质粒pNW102转导至枯草芽孢杆菌BF 7658中。将枯草芽孢杆菌BF7658 (pNW102) 菌株在非允许温度下长时间处理，获得了许多重组菌株。这是pNW102的α-淀粉酶基因与BF7658之间的同源重组结果。同源重组发生在α-淀粉酶基因的任何位点，但存在一些热点。我们已经从重组菌株中筛选出2株能够在枯草芽孢杆菌BF7658中稳定产生耐热α-淀粉酶的菌株。酶学分析表明，重组菌株产生的耐热α-淀粉酶的特性与地衣芽孢杆菌的相同。
