Speranza Antonio, Ortosecco Giovanni, Castaldi Elena, Nardelli Anna, Pace Leonardo, Salvatore Marco
IBB-CNR, Edificio 10, Via Pansini 5, Napoli, Italy.
Appl Radiat Isot. 2009 Sep;67(9):1664-9. doi: 10.1016/j.apradiso.2009.04.008. Epub 2009 Apr 22.
Automatic synthesis of 4-[18F]fluorobenzaldehyde has been developed by a commercially available TRACERlab FX(F-N) synthesis module to be used as prosthetic group for amino-oxy functionalized peptide labelling in clinical routine application. In addition a handmade purification device (HPD) has been setup to perform automatic cartridge purification as well as to back-up the reactor where one-pot synthesis is not applicable. Cartridges for solid phase extraction such as C18, C8, phenyl has been tested to best perform purification as well as activity recovery. Radiochemical yield (RCY) at end of synthesis (EOS) was in average 67% after about 45 min (90% decay corrected at EOB). The RCY of the entire procedure was 54% with a radiochemical purity above 99%.
通过市售的TRACERlab FX(F-N)合成模块开发了4-[18F]氟苯甲醛的自动合成方法，用于临床常规应用中氨基氧基功能化肽标记的辅基。此外，还设置了一个手工制作的纯化装置(HPD)，以进行自动柱纯化，并在一锅法合成不适用时作为反应器的备用装置。已测试了用于固相萃取的柱，如C18、C8、苯基柱，以实现最佳的纯化和活性回收。合成结束时(EOS)的放射化学产率(RCY)在约45分钟后平均为67%(EOB时经90%衰变校正)。整个过程的RCY为54%，放射化学纯度高于99%。
