Nakajima Masanobu, Kato Hiroyuki, Miyazaki Tatsuya, Fukuchi Minoru, Masuda Norihiro, Fukai Yasuyuki, Sohda Makoto, Ahmad Faried, Kuwano Hiroyuki
Department of General Surgical Science (Surgery I), Gunma University, Graduate School of Medicine, 3-39-22, Showa-machi, Maebashi, Gunma 371-8511, Japan.
Anticancer Res. 2009 May;29(5):1595-606.
The objective of this study was to clarify the participation of heat-shock protein 70 (HSP70) and the humoral immune system in antitumor immunity in esophageal cancer.
Immunohistochemical staining for HSP 70, and CD4(+) T-, CD8(+) T-, B- and plasma cells was performed on surgical specimens obtained from 125 patients with esophageal cancer. An enzyme-linked immunosorbent assay (ELISA) was then performed to measure serum anti-HSP70 antibodies in the azygos vein.
The expression of HSP 70 correlated inversely with depth of invasion (p<0.0001), pathological stage (p<0.0001) and blood vessel invasion (p<0.001), and there was a positive correlation between HSP70 and CD4(+) T-, CD8(+) T-, B- and plasma cells. Of these, the B- and plasma cells had the strongest correlation to HSP70 expression. Serum anti-HSP70 antibody levels in the azygos vein correlated with HSP70 expression, and B and plasma cell infiltration. Patients positive for HSP70, and B- and plasma cell infiltration had good prognosis compared to other cases. According to multivariate analyses, simultaneous occurrence of HSP70 expression, and B- and plasma cell infiltration is a stronger prognostic factor than simultaneous occurrence of HSP70 expression, and CD4(+) T- and CD8(+) T-cell infiltration.
It is suggested that the HSP70-humoral immune cell system might play an important role in antitumor effects in patients with esophageal cancer.
本研究的目的是阐明热休克蛋白70（HSP70）和体液免疫系统在食管癌抗肿瘤免疫中的作用。
对125例食管癌患者手术标本进行HSP 70、CD4(+) T细胞、CD8(+) T细胞、B细胞和浆细胞的免疫组织化学染色。然后进行酶联免疫吸附测定（ELISA），以测量奇静脉中的血清抗HSP70抗体。
HSP 70的表达与浸润深度（p<0.0001）、病理分期（p<0.0001）和血管浸润（p<0.001）呈负相关，HSP70与CD4(+) T细胞、CD8(+) T细胞、B细胞和浆细胞呈正相关。其中，B细胞和浆细胞与HSP70表达的相关性最强。奇静脉中的血清抗HSP70抗体水平与HSP70表达及B细胞和浆细胞浸润相关。HSP70阳性以及B细胞和浆细胞浸润的患者预后优于其他病例。多因素分析显示，HSP70表达与B细胞和浆细胞浸润同时出现是比HSP70表达与CD4(+) T细胞和CD8(+) T细胞浸润同时出现更强的预后因素。
提示HSP70-体液免疫细胞系统可能在食管癌患者的抗肿瘤作用中发挥重要作用。
