Herz Itzhak, Rosso Raphael, Roth Arie, Keren Gad, George Jacob
The Department of Cardiology, Tel Aviv Sourasky Medical Center and The Sackler Faculty of Medicine, Tel Aviv University, Israel.
Acute Card Care. 2006;8(1):46-50. doi: 10.1080/14628840600606950.
The heat shock protein (HSP) family comprises approximately 24 proteins displaying a high degree of sequence homology between different species. The induction of self-HSP immune reactivity is thought to be involved in the pathogenesis of several diseases. Antibodies to HSP60/65 have been demonstrated in the sera of patients with coronary artery disease. Moreover, the target antigens of the antibodies HSP60 and HSP70 are both expressed in smooth muscle cells and macrophages within atherosclerotic lesions. In this retrospective, case control study, we investigated whether patients with established atherosclerosis, with either stable or unstable angina have high levels of antibodies to HSP70.
Patients with stable angina (n = 40) were from the outpatient clinic whereas patients with unstable angina (n = 91) were recruited upon admission and prior to performance of coronary angiography. Control patients (n = 18) were healthy subjects with no evidence of coronary artery disease. Serum levels of anti-HSP70 antibodies were assayed by ELISA. Patients with stable and unstable angina exhibited lower serum levels of antibodies to HSP70 (0.202+/-0.113 and 0.201+/-0.115, respectively) in comparison to control subjects (0.364+/-0.199, P = 0.0001 for both comparisons). Serum levels of antibodies to HSP70 did not differ significantly between patients with stable and unstable angina. No differences in serum levels of antibodies to HSP70 were evident between baseline and follow up in the patients with unstable angina.
Patients with coronary atherosclerosis possess lower levels of anti-HSP70 antibody levels. Further research is required to explore whether higher levels of anti-HSP70 antibodies have a predictive value in coronary atherosclerosis.
热休克蛋白（HSP）家族由大约24种蛋白质组成，不同物种间这些蛋白质具有高度的序列同源性。自身HSP免疫反应性的诱导被认为与多种疾病的发病机制有关。在冠心病患者的血清中已证实存在针对HSP60/65的抗体。此外，抗体HSP60和HSP70的靶抗原均在动脉粥样硬化病变内的平滑肌细胞和巨噬细胞中表达。在这项回顾性病例对照研究中，我们调查了患有稳定性或不稳定性心绞痛的已确诊动脉粥样硬化患者是否具有高水平的抗HSP70抗体。
稳定性心绞痛患者（n = 40）来自门诊，而不稳定性心绞痛患者（n = 91）在入院时及冠状动脉造影术前被招募。对照患者（n = 18）为无冠状动脉疾病证据的健康受试者。采用酶联免疫吸附测定法（ELISA）检测血清抗HSP70抗体水平。与对照受试者（0.364±0.199）相比，稳定性和不稳定性心绞痛患者的血清抗HSP70抗体水平较低（分别为0.202±0.113和0.201±0.115，两项比较P = 0.0001）。稳定性和不稳定性心绞痛患者之间血清抗HSP70抗体水平无显著差异。不稳定性心绞痛患者在基线和随访之间血清抗HSP70抗体水平无明显差异。
冠状动脉粥样硬化患者的抗HSP70抗体水平较低。需要进一步研究以探讨更高水平的抗HSP70抗体在冠状动脉粥样硬化中是否具有预测价值。
