Bender M A, Moore R C
Medical Department, Brookhaven National Laboratory, Upton, NY 11973.
Mutat Res. 1991 Sep-Oct;250(1-2):319-24. doi: 10.1016/0027-5107(91)90187-s.
Earlier work of several laboratories established that the yields of radiation-induced ring and dicentric chromosomes are greater when human peripheral blood lymphocytes are irradiated in GH1 some hours after phytohemagglutinin stimulation than if they are irradiated in G0 before stimulation. Post-treatment of lymphocytes irradiated in G0 with the DNA polymerase inhibitor aphidicolin, which is effective against both pol alpha and pol delta, produces a similar increase in ring and dicentric yield. We found that aphidicolin post-treatment was much less effective in increasing ring and dicentric yield increases in cells irradiated in G1 four to five hours after stimulation. Because we had earlier found specific inhibitors of DNA pol alpha ineffective in producing increased yields in either G0 or G1 lymphocytes, we conclude that much of the G0 to G1 increase in yields is mediated by pol delta.
几个实验室早期的研究表明，在植物血凝素刺激数小时后的GH1期照射人外周血淋巴细胞时，辐射诱导的环状和双着丝粒染色体的产量要高于在刺激前的G0期照射时。用对α-聚合酶和δ-聚合酶均有效的DNA聚合酶抑制剂阿非迪霉素对在G0期照射的淋巴细胞进行处理后，环状和双着丝粒染色体的产量也会有类似的增加。我们发现，阿非迪霉素处理对刺激后4至5小时处于G1期照射的细胞增加环状和双着丝粒染色体产量的效果要差得多。因为我们之前发现DNAα-聚合酶的特异性抑制剂对G0期或G1期淋巴细胞产量的增加均无效，所以我们得出结论，产量从G0期到G1期的大幅增加是由δ-聚合酶介导的。
