Bender M A, Moore R C
Medical Department, Brookhaven National Laboratory, Upton, NY 11973.
Mutat Res. 1990 Jun;244(2):111-4. doi: 10.1016/0165-7992(90)90058-r.
We report experiments to test the hypothesis that the increased yield of dicentric chromosomes observed in human peripheral blood lymphocytes treated with X-rays during the G1 phase of their first cell cycle, as compared with the yield when the cells are treated in their G0 phase prior to phytohemagglutinin stimulation, is a manifestation of the recently-reported conversion of an inactive form of DNA polymerase alpha to its active form as the PHA-stimulated cells pass from G0 into G1 (Sylvia et al., 1988). The specific polymerase alpha inhibitor butylphenyl deoxyguanosine was used as an X-ray post-treatment. The results show that polymerase alpha is not involved.
我们报告了一些实验，以检验以下假设：在人类外周血淋巴细胞第一个细胞周期的G1期用X射线处理时，与在植物血凝素刺激前处于G0期时处理细胞相比，观察到的双着丝粒染色体产量增加，是最近报道的随着植物血凝素刺激的细胞从G0期进入G1期，DNA聚合酶α的无活性形式向其活性形式转变的一种表现（西尔维亚等人，1988年）。特定的聚合酶α抑制剂丁基苯基脱氧鸟苷被用作X射线处理后的药物。结果表明，聚合酶α并不参与其中。
