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氟苯达唑和伊维菌素联合体外治疗对细粒棘球绦虫原头节和囊尾蚴有显著效果。

Flubendazole and ivermectin in vitro combination therapy produces a marked effect on Echinococcus granulosus protoscoleces and metacestodes.

作者信息

Elissondo M Celina, Ceballos L, Alvarez L, Sánchez Bruni S, Lanusse C, Denegri G

机构信息

Laboratorio de Zoonosis Parasitarias, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3250, 7600, Mar del Plata, Argentina.

出版信息

Parasitol Res. 2009 Sep;105(3):835-42. doi: 10.1007/s00436-009-1469-y. Epub 2009 May 15.

DOI:10.1007/s00436-009-1469-y
PMID:19444468
Abstract

The aim of the present work was to evaluate the in vitro efficacy of the flubendazole (FLBZ) and ivermectin (IVM) combination against Echinococcus granulosus protoscoleces and metacestodes. Protoscoleces and groups of ten peritoneal cysts obtained from BALB/c mice were incubated with the two drugs, either separately or in combination, at the following final concentrations: 10 microg/mL FLBZ, 1 microg/mL FLBZ, 1 microg/mL IVM, 10 microg/mL FLBZ + 1 microg/mL IVM, and 1 microg/mL FLBZ + 1 microg/mL IVM. The maximum protoscolicidal effect was found with the combination 10 microg/mL FLBZ + 1 microg/mL IMV. After 1 day of incubation, the presence of numerous blebs in the tegument of protoscoleces was observed. Ultrastructural studies revealed that the primary site of damage was the tegument of the parasite. The effect of the two drugs on hydatid cysts obtained from mice was more rapidly detected in cysts treated with the combination of FLBZ + IVM than when drugs were used separately. Ultrastructural studies revealed that the germinal layer of treated cysts lost the multicellular structure feature and underwent considerable degenerative changes after in vitro treatment. The outcomes obtained demonstrated the favorable effect of the combination of FLBZ and IVM against E. granulosus.

摘要

本研究的目的是评估氟苯达唑（FLBZ）和伊维菌素（IVM）联合使用对细粒棘球绦虫原头节和幼虫的体外疗效。从BALB/c小鼠获得的原头节和十组腹膜囊肿分别或联合用以下终浓度的两种药物孵育：10μg/mL氟苯达唑、1μg/mL氟苯达唑、1μg/mL伊维菌素、10μg/mL氟苯达唑 + 1μg/mL伊维菌素以及1μg/mL氟苯达唑 + 1μg/mL伊维菌素。发现10μg/mL氟苯达唑 + 1μg/mL伊维菌素联合使用时原头节杀伤效果最佳。孵育1天后，观察到原头节体表有大量泡状突起。超微结构研究显示，损伤的主要部位是寄生虫的体表。与单独使用药物相比，氟苯达唑 + 伊维菌素联合处理的小鼠包虫囊肿对两种药物的作用检测得更快。超微结构研究显示，体外处理后，处理过的囊肿生发层失去了多细胞结构特征，并发生了相当程度的退行性变化。所获得的结果证明了氟苯达唑和伊维菌素联合使用对细粒棘球绦虫具有良好疗效。

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