Karpisheh Elaheh, Sadjjadi Seyed Mahmoud, Nekooeian Ali Akbar, Sharifi Yosef
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, P.O. Box 71345-1735, Shiraz, Iran.
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
J Parasit Dis. 2023 Dec;47(4):850-858. doi: 10.1007/s12639-023-01632-4. Epub 2023 Nov 13.
The present study was aimed to assess the structural changes in protoscoleces of sensu stricto following exposure to different natural and chemical protoscolicidal agents using differential interference contrast (DIC)/Nomarski microscopy. Protoscoleces of sheep's liver cysts were collected aseptically. Individually, about 1000 protoscoleces were exposed to 0.5% silver nitrate, 20% hypertonic saline solution, 0.5% cetrimide solution and two different concentrations of garlic chloroformic extraction as well as phosphate-buffered saline (PBS). The protoscoleces viability was assessed using 0.1% eosin solution, and structural modifications in the protoscoleces were examined by DIC/Nomarski microscopy. The results revealed the degeneration of the tegument, disorganization of the hooks, and reduction of the size of the protoscoleces exposed to cetrimide, hypertonic sodium chloride, and silver nitrate. Furthermore, calcareous corpuscles became blurred and opaque and their numbers decreased in all the exposed samples except, those in PBS. The exposed protoscoleces to cetrimide and hypertonic sodium chloride solution showed extensive degeneration of the tegument and disorganization of the hooks. In the group exposed to 200 mg/ml chloroformic garlic extract, the protoscoleces' width decreased. The length, width, and number of calcareous corpuscles also decreased significantly in the silver nitrate-exposed protoscoleces. The study concludes that protoscoleces exposed to different solutions; cetrimide 0.5% and hypertonic sodium chloride 20% caused more pronounced structural changes in the exposed protoscoleces. These changes were well demonstrated by DIC microscopy and can be used as a supplementary tool to evaluate the effects of protoscolicidal agents.
The online version contains supplementary material available at 10.1007/s12639-023-01632-4.
本研究旨在利用微分干涉对比（DIC）/诺马斯基显微镜评估细粒棘球绦虫原头节在暴露于不同天然和化学灭原头节剂后的结构变化。无菌收集绵羊肝囊肿的原头节。分别将约1000个原头节暴露于0.5%硝酸银、20%高渗盐溶液、0.5%西曲溴铵溶液、两种不同浓度的大蒜氯仿提取物以及磷酸盐缓冲盐水（PBS）中。使用0.1%伊红溶液评估原头节活力，并通过DIC/诺马斯基显微镜检查原头节的结构改变。结果显示，暴露于西曲溴铵、高渗氯化钠和硝酸银的原头节出现皮层退化、钩的结构紊乱以及原头节尺寸减小。此外，除PBS处理的样本外，所有暴露样本中的石灰小体均变得模糊不清且数量减少。暴露于西曲溴铵和高渗氯化钠溶液的原头节显示出广泛的皮层退化和钩的结构紊乱。在暴露于200mg/ml大蒜氯仿提取物的组中，原头节宽度减小。暴露于硝酸银的原头节中，石灰小体的长度、宽度和数量也显著减少。研究得出结论，暴露于不同溶液（0.5%西曲溴铵和20%高渗氯化钠）的原头节在暴露的原头节中引起更明显的结构变化。这些变化通过DIC显微镜得到了很好的证明，可作为评估灭原头节剂效果的辅助工具。
在线版本包含可在10.1007/s12639-023-01632-4获取的补充材料。
