Kranaster Ramon, Marx Andreas
Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
Angew Chem Int Ed Engl. 2009;48(25):4625-8. doi: 10.1002/anie.200900953.
A novel screening approach based on an oligonucleotide-addressing enzyme assay enables multiplexed simultaneous profiling of DNA polymerases in nanoliter volumes in terms of their different properties. This approach was used to identify enzymes with altered properties out of a library of protein mutants.
一种基于寡核苷酸寻址酶分析的新型筛选方法，能够在纳升体积内根据不同特性对DNA聚合酶进行多重同时分析。该方法用于从蛋白质突变体文库中鉴定出特性发生改变的酶。
