Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Angew Chem Int Ed Engl. 2010 Aug 9;49(34):5921-4. doi: 10.1002/anie.201001607.
[Image: see text] We present the application of an activity-based phage display method to identify DNA polymerases tailored for next generation sequencing applications. Using this approach, we identify a mutant of Taq DNA polymerase that incorporates the fluorophore-labeled dA, dT, dC, and dG substrates ~50 to 400-fold more efficiently into scarred primers in solution and that also demonstrates significantly improved performance under actual sequencing conditions.
[图片：见正文]我们展示了一种基于活性的噬菌体展示方法在鉴定为下一代测序应用定制的 DNA 聚合酶方面的应用。通过这种方法，我们鉴定出了一种 Taq DNA 聚合酶的突变体，它能将标记有荧光基团的 dA、dT、dC 和 dG 底物在溶液中更有效地掺入有缺口的引物中，效率提高了 50 到 400 倍，并且在实际测序条件下也表现出了显著改善的性能。
