Guthrie P B, Segal M, Kater S B
Department of Anatomy and Neurobiology, Colorado State University, Fort Collins.
Nature. 1991 Nov 7;354(6348):76-80. doi: 10.1038/354076a0.
The dendritic spine is a basic structural unit of neuronal organization. It is assumed to be a primary locus of synaptic plasticity, and to undergo long-term morphological and functional changes, at least some of which are regulated by intracellular calcium concentrations. It is known that physiological stimuli can cause marked increases in intracellular calcium levels in hippocampal dendritic shafts, but it is completely unknown to what extent such changes in the dendrites would also be seen by calcium-sensing structures within spines. Will calcium levels in all spines change in parallel with the dendrite or will there be a heterogeneous response? This study, through direct visualization and measurement of intracellular calcium concentrations in individual living spines, demonstrates that experimentally evoked changes in calcium concentrations in the dendritic shaft ([Ca2+]d).
树突棘是神经元组织的基本结构单位。它被认为是突触可塑性的主要位点，并经历长期的形态和功能变化，其中至少一些变化受细胞内钙浓度调节。已知生理刺激可导致海马树突轴内细胞内钙水平显著升高，但完全不清楚树突中的这种变化在棘内的钙传感结构中会在多大程度上也能被观察到。所有棘中的钙水平会与树突平行变化还是会有不同的反应？本研究通过直接可视化和测量单个活棘内的细胞内钙浓度，证明了实验诱发的树突轴内钙浓度（[Ca2+]d）的变化。
