Kirkeby S, Martel C J M, Aasted B
Dental School, Faculty of Health Sciences, University of Copenhagen, Denmark.
Virus Res. 2009 Sep;144(1-2):225-32. doi: 10.1016/j.virusres.2009.05.004. Epub 2009 May 15.
Glycans terminating in sialic acids serve as receptors for influenza viruses. In this study ferrets were infected with influenza virus A/New Caledonia/20/99, and the in situ localization of sialic acids linked alpha2-3 and alpha2-6 in the airways was investigated in infected and non-infected animals by use of sialic acid detecting lectins and a monoclonal antibody towards the Sialyl-Tn antigen. The goblet cells in the bronchi from non-infected ferrets expressed Sialyl alpha 2-6Gal glycans, while the seromucinous glands in the submucosa expressed Sialyl alpha 2-3Gal glycans. In the infected animals, the surface epithelial cells in some bronchi showed metaplasia and expressed the Sialyl-Tn antigen: Sialyl alpha 2-6GalNAc-O-Thr/Ser. The submucosal tracheal glands in these animals showed increased expression of both Sialyl alpha 2-3 and Sialyl alpha 2-6 epitopes.
以唾液酸为末端的聚糖可作为流感病毒的受体。在本研究中，雪貂感染了甲型流感病毒/新喀里多尼亚/20/99，通过使用唾液酸检测凝集素和针对唾液酸化Tn抗原的单克隆抗体，对感染和未感染动物气道中α2-3和α2-6连接的唾液酸进行原位定位研究。未感染雪貂支气管中的杯状细胞表达唾液酸α2-6Gal聚糖，而黏膜下层的浆液黏液腺表达唾液酸α2-3Gal聚糖。在感染动物中，一些支气管的表面上皮细胞出现化生并表达唾液酸化Tn抗原：唾液酸α2-6GalNAc-O-Thr/Ser。这些动物的气管黏膜下腺中唾液酸α2-3和唾液酸α2-6表位的表达均增加。
