Babal P, Slugen I, Danis D, Zaviacic M, Gardner W A
Department of Pathology, University of South Alabama, Mobile 36617, USA.
Acta Histochem. 1996 Jan;98(1):71-7.
Three sialic acid-specific lectins, Tritrichomonas mobilensis (TML) (without linkage preference), Maackia amurensis leukoagglutinin (MAL) (alpha2,3) and Sambucus nigra agglutinin (alpha2,6 linkage-specific), were used for detection of sialylated glycoconjugates in normal and diseased human kidneys. Normal kidneys demonstrated strong podocyte positivity of alpha2,3 linked sialic acid and weaker sialic acid expression on capillary endothelium, which was alpha2,6 linked. Renal biopsies (45) representing a variety of diseases, e.g. minimal change disease, membranous, membranoproliferative glomerulonephritis, tubulointerstitial nephrosis or diabetic glomerulopathy showed increased sialic acid expression in glomerular capillaries, Bowman's capsule epithelium and on podocytes. In several different kidney diseases the glomerular endothelium expressed also alpha2,3 linked sialic acid along with increased TML-positivity of epithelial cells related to alpha2,6 linked sialic acid. No difference in sialic acid linkage expression was observed in the tubules, which expressed putative alpha2,6 linked sialic acid on the luminal surface of cells distal to the descending limb of Henle's loop. The study did not show changes characteristic of specific diseases. Rather, increased sialic acid expression on glomerular endothelium and podocytes was linked to a variety of pathological changes. It is assumed that changes in sialysation of glycoconjugates in the glomeruli represent nonspecific changes and do not reflect fundamental pathogenetic features of renal diseases.
三种唾液酸特异性凝集素，即活动三毛滴虫凝集素（TML）（无连接偏好）、黑果接骨木凝集素（MAL）（α2,3）和黑接骨木凝集素（α2,6连接特异性），用于检测正常和患病人类肾脏中的唾液酸化糖缀合物。正常肾脏显示α2,3连接的唾液酸在足细胞中有强阳性，而在毛细血管内皮上的唾液酸表达较弱，其为α2,6连接。代表多种疾病的45份肾活检标本，如微小病变病、膜性肾病、膜增生性肾小球肾炎、肾小管间质性肾病或糖尿病性肾小球病，显示肾小球毛细血管、鲍曼囊上皮和足细胞中的唾液酸表达增加。在几种不同的肾脏疾病中，肾小球内皮也表达α2,3连接的唾液酸，同时与α2,连接的唾液酸相关的上皮细胞的TML阳性增加。在肾小管中未观察到唾液酸连接表达的差异，在亨氏袢降支远端细胞的腔面表达推测为α2,6连接的唾液酸。该研究未显示特定疾病的特征性变化。相反，肾小球内皮和足细胞上唾液酸表达的增加与多种病理变化有关。据推测，肾小球中糖缀合物唾液酸化的变化代表非特异性变化，并不反映肾脏疾病的基本发病机制特征。
